Overview

  • Product nameAnti-Granulocytes antibody [HIS48] (Biotin)
    See all Granulocytes primary antibodies
  • Description
    Mouse monoclonal [HIS48] to Granulocytes (Biotin)
  • ConjugationBiotin
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Rat bone marrow cells

  • Positive control
    • Rat splenocyte suspensions
  • General notes


    ab95747 is commonly used as a phenotypic marker for Rat granulocytes.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS, 500mM Sodium chloride, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • Primary antibody notesab95747 is commonly used as a phenotypic marker for Rat granulocytes.
  • ClonalityMonoclonal
  • Clone numberHIS48
  • IsotypeIgM
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab95747 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.06µg for 105-8 cells. A 100 µl volume is recommended for staining the cell sample.



ab18396-Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.

Target

  • RelevanceGranulocytes are a type of white blood cell filled with microscopic granules that are little sacs containing enzymes, compounds that digest microorganisms. They are part of the innate immune system and have somewhat nonspecific, broad-based activity. They do not respond exclusively to specific antigens, as do B-cells and T-cells. Neutrophils, eosinophils and basophils are all types of granulocytes. They are named by the staining features of their granules in the laboratory: Neutrophils have "neutral" subtle granules; Eosinophils have prominent granules that stain readily with the acid dye eosin; and Basophils have prominent granules that stain readily basic (non acidic) dyes.

References for Anti-Granulocytes antibody [HIS48] (Biotin) (ab95747)

This product has been referenced in:
  • Rhodes NP  et al. Macrophage subpopulation differentiation by stimulation with biomaterials. J Biomed Mater Res 37:481-8 (1997). Read more (PubMed: 9407296) »
  • Badger DA  et al. The role of inflammatory cells and cytochrome P450 in the potentiation of CCl4-induced liver injury by a single dose of retinol. Toxicol Appl Pharmacol 141:507-19 (1996). Read more (PubMed: 8975775) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"