The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.06µg for 105-8 cells. A 100 µl volume is recommended for staining the cell sample.
ab18396-Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.
RelevanceGranulocytes are a type of white blood cell filled with microscopic granules that are little sacs containing enzymes, compounds that digest microorganisms. They are part of the innate immune system and have somewhat nonspecific, broad-based activity. They do not respond exclusively to specific antigens, as do B-cells and T-cells.
Neutrophils, eosinophils and basophils are all types of granulocytes. They are named by the staining features of their granules in the laboratory: Neutrophils have "neutral" subtle granules; Eosinophils have prominent granules that stain readily with the acid dye eosin; and Basophils have prominent granules that stain readily basic (non acidic) dyes.
References for Anti-Granulocytes antibody [HIS48] (Biotin) (ab95747)
This product has been referenced in:
Rhodes NP et al. Macrophage subpopulation differentiation by stimulation with biomaterials. J Biomed Mater Res37:481-8 (1997).
Read more (PubMed: 9407296) »
Badger DA et al. The role of inflammatory cells and cytochrome P450 in the potentiation of CCl4-induced liver injury by a single dose of retinol. Toxicol Appl Pharmacol141:507-19 (1996).
Read more (PubMed: 8975775) »