Overview

  • Product nameAnti-Granulocytes antibody [HIS48] (FITC)
    See all Granulocytes primary antibodies
  • Description
    Mouse monoclonal [HIS48] to Granulocytes (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • SpecificityReacts with a molecule expressed on the surface of all rat granulocytes. The antigen recognized by ab93549 is also expressed by erythroid lineage cells during their developmental stages in the bone marrow.
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Positive control
    • Rat splenocytes
  • General notes


    Clone HIS48 is commonly used as a phenotypic marker for rat granulocytes.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS, 500mM Sodium chloride, pH 7.2
  • Concentration information loading...
  • PurityPurified IgM
  • Primary antibody notesClone HIS48 is commonly used as a phenotypic marker for rat granulocytes.
  • ClonalityMonoclonal
  • Clone numberHIS48
  • IsotypeIgM
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab93549 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.25µg for 105-8 cells.

Use 0.25µg for 105 - 108 cells in a final volume of 100 µl.





ab91546-Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.

Target

  • RelevanceGranulocytes are a type of white blood cell filled with microscopic granules that are little sacs containing enzymes, compounds that digest microorganisms. They are part of the innate immune system and have somewhat nonspecific, broad-based activity. They do not respond exclusively to specific antigens, as do B-cells and T-cells. Neutrophils, eosinophils and basophils are all types of granulocytes. They are named by the staining features of their granules in the laboratory: Neutrophils have "neutral" subtle granules; Eosinophils have prominent granules that stain readily with the acid dye eosin; and Basophils have prominent granules that stain readily basic (non acidic) dyes.

References for Anti-Granulocytes antibody [HIS48] (FITC) (ab93549)

This product has been referenced in:
  • Rhodes NP  et al. Macrophage subpopulation differentiation by stimulation with biomaterials. J Biomed Mater Res 37:481-8 (1997). Read more (PubMed: 9407296) »
  • Badger DA  et al. The role of inflammatory cells and cytochrome P450 in the potentiation of CCl4-induced liver injury by a single dose of retinol. Toxicol Appl Pharmacol 141:507-19 (1996). Read more (PubMed: 8975775) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"