Overview

  • Product nameAnti-Granzyme A antibody [GA6]
    See all Granzyme A primary antibodies
  • Description
    Mouse monoclonal [GA6] to Granzyme A
  • Tested applicationsSuitable for: IHC-FoFr, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant Granzyme A (Human).

Properties

Applications

Our Abpromise guarantee covers the use of ab10870 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration. PubMed: 19827750
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Predicted molecular weight: 31.4 kDa.

Recognises a 60kD disulphide-linked homodimeric protein.

Target

  • FunctionThis enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Lys or Arg. Cleaves APEX1 after 'Lys-31' and destroys its oxidative repair activity. Involved in apoptosis.
  • Sequence similaritiesBelongs to the peptidase S1 family. Granzyme subfamily.
    Contains 1 peptidase S1 domain.
  • Cellular localizationSecreted. Cytoplasmic granule.
  • Information by UniProt
  • Database links
  • Alternative names
    • CTL tryptase antibody
    • CTLA3 antibody
    • Cytolytic t cell and natural killer cell specific trypsin like serine protease antibody
    • Cytotoxic T lymphocyte associated serine esterase 3 antibody
    • Cytotoxic T lymphocyte proteinase 1 antibody
    • Cytotoxic T-lymphocyte proteinase 1 antibody
    • Fragmentin 1 antibody
    • Fragmentin-1 antibody
    • GRAA_HUMAN antibody
    • Granzyme 1 cytotoxic T lymphocyte associated serine esterase 3 antibody
    • Granzyme A (Cytotoxic T lymphocyte associated serine esterase 3; Hanukah factor serine protease) antibody
    • Granzyme A (granzyme 1, cytotoxic T lymphocyte associated serine esterase 3) antibody
    • Granzyme A antibody
    • Granzyme A precursor antibody
    • Granzyme-1 antibody
    • GZMA antibody
    • H factor antibody
    • Hanukah factor serine protease antibody
    • Hanukkah factor antibody
    • HF antibody
    • HFSP antibody
    • TSP1 antibody
    see all

Anti-Granzyme A antibody [GA6] images

  • ab10870 staining Granzyme A in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formalin and blocked with 10% serum for 30 minutes at 24°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/50 in horse serum) for 16 hours at 4°C. A Biotin-conjugated horse anti-mouse IgG polyclonal (1/375) was used as the secondary antibody.

    See Abreview

References for Anti-Granzyme A antibody [GA6] (ab10870)

This product has been referenced in:
  • Li H  et al. Glucocorticoid Receptor and Sequential P53 Activation by Dexamethasone Mediates Apoptosis and Cell Cycle Arrest of Osteoblastic MC3T3-E1 Cells. PLoS One 7:e37030 (2012). WB ; Mouse . Read more (PubMed: 22719835) »
  • Hirono S  et al. Molecular markers associated with lymph node metastasis in pancreatic ductal adenocarcinoma by genome-wide expression profiling. Cancer Sci : (2009). IHC-FoFr ; Human . Read more (PubMed: 19817750) »

See all 2 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer
Sample Human Tissue sections (heart)
Specification heart
Permeabilization No
Fixative formalin
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Submitted Oct 29 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"