Overview

  • Product name
  • Description
    Rabbit polyclonal to Granzyme B
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human Granzyme B.

    (Peptide available as ab73723.)

  • Positive control
    • This antibody gave a positive signal in the following tissue lysates: Pancreas (Mouse); Pancreas (Rat)

Properties

Applications

Our Abpromise guarantee covers the use of ab73415 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).

Target

  • Function
    This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.
  • Sequence similarities
    Belongs to the peptidase S1 family. Granzyme subfamily.
    Contains 1 peptidase S1 domain.
  • Cellular localization
    Cytoplasmic granule. Cytoplasmic granules of cytolytic T-lymphocytes and natural killer cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • C11 antibody
    • Cathepsin G like 1 antibody
    • Cathepsin G-like 1 antibody
    • CCPI antibody
    • CGL 1 antibody
    • CGL1 antibody
    • CSP B antibody
    • CSPB antibody
    • CTLA-1 antibody
    • CTLA1 antibody
    • CTSGL1 antibody
    • Cytotoxic serine protease B antibody
    • Cytotoxic T lymphocyte associated serine esterase 1 antibody
    • Cytotoxic T lymphocyte proteinase 2 antibody
    • Cytotoxic T-lymphocyte proteinase 2 antibody
    • Fragmentin 2 antibody
    • Fragmentin-2 antibody
    • GRAB_HUMAN antibody
    • Granzyme 2 antibody
    • Granzyme B (granzyme 2, cytotoxic T lymphocyte associated serine esterase 1) antibody
    • Granzyme B antibody
    • Granzyme-2 antibody
    • GranzymeB antibody
    • GRB antibody
    • Gzmb antibody
    • Hlp antibody
    • Human lymphocyte protein antibody
    • Lymphocyte protease antibody
    • Protease, serine, B antibody
    • SECT antibody
    • T cell serine protease 1-3E antibody
    • T-cell serine protease 1-3E antibody
    see all

Images

  • All lanes : Anti-Granzyme B antibody (ab73415) at 1 µg/ml

    Lane 1 : Pancreas (Mouse) Tissue Lysate
    Lane 2 : Pancreas (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 28 kDa
    Observed band size : 28 kDa
    Additional bands at : 24 kDa,32 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute

References

ab73415 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab73415.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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