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Recombinant fragment, corresponding to amino acids 7-227 of Human Granzyme B.
Our Abpromise guarantee covers the use of ab17929 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration. ab91356-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
Detection of granzyme B in permeabilized human CD8+ T lymphocytes (filled profile). Isotype control in combination with a secondary FITC-labeled antibody is shown as control.
Method: Permeabilized human T cells (5 x 105) were incubated on ice for 30 min in 50
Detection of granzyme B in a human CD8+ T cell clone.
Method: Human T cells were plated onto polylysine treated glass slides, fixed and permeabilized in methanol at -20oC for 5 min, then in acetone at -20oC for 30 sec. After 3 washes in PBS, 0.1% BSA, slides were incubated for 1 hr at room temperature (RT) with 20
Lane 1: 40 mg of lysate
Lane 2: 4 mg of lysate
Lane 3: 0.4 mg of lysate
Method: Recombinant granzyme B was resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the monoclonal anti-granzyme B non-conjugated form of antibody ab17929 at 1µg/ml. Proteins were visualized using a peroxidase-conjugated antibody to mouse IgG (SBA) and a chemiluminescence detection system.