Overview

  • Product nameAnti-Granzyme B antibody [GB11]
    See all Granzyme B primary antibodies
  • Description
    Mouse monoclonal [GB11] to Granzyme B
  • Tested applicationsSuitable for: Flow Cyt, IHC-Fr, IPmore details
  • Species reactivity
    Reacts with: Human, Chimpanzee, Monkey, Rhesus monkey
  • Immunogen

    Full length native protein (purified) (Human).

  • General notesThis product should be stored undiluted. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

Properties

Applications

Our Abpromise guarantee covers the use of ab10912 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
IHC-Fr
IP
  • Application notesFlow Cyt: 1/10 - 1/100. Membrane permeabilization is required for flow cytometry. The use of LeucopermTM is recommended for this purpose. Use 10µl of the suggested working dilution to label 106 cells or 100ul whole blood.
    IHC-Fr: Use at an assay dependent dilution. Hsi, B-L. et al. (1986)
    IP: Use at an assay dependent dilution.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionThis enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.
    • Sequence similaritiesBelongs to the peptidase S1 family. Granzyme subfamily.
      Contains 1 peptidase S1 domain.
    • Cellular localizationCytoplasmic granule. Cytoplasmic granules of cytolytic T-lymphocytes and natural killer cells.
    • Information by UniProt
    • Database links
    • Alternative names
      • C11 antibody
      • Cathepsin G like 1 antibody
      • Cathepsin G-like 1 antibody
      • CCPI antibody
      • CGL 1 antibody
      • CGL1 antibody
      • CSP B antibody
      • CSPB antibody
      • CTLA-1 antibody
      • CTLA1 antibody
      • CTSGL1 antibody
      • Cytotoxic serine protease B antibody
      • Cytotoxic T lymphocyte associated serine esterase 1 antibody
      • Cytotoxic T lymphocyte proteinase 2 antibody
      • Cytotoxic T-lymphocyte proteinase 2 antibody
      • Fragmentin 2 antibody
      • Fragmentin-2 antibody
      • GRAB_HUMAN antibody
      • Granzyme 2 antibody
      • Granzyme B (granzyme 2, cytotoxic T lymphocyte associated serine esterase 1) antibody
      • Granzyme B antibody
      • Granzyme-2 antibody
      • GranzymeB antibody
      • GRB antibody
      • Gzmb antibody
      • Hlp antibody
      • Human lymphocyte protein antibody
      • Lymphocyte protease antibody
      • Protease, serine, B antibody
      • SECT antibody
      • T cell serine protease 1-3E antibody
      • T-cell serine protease 1-3E antibody
      see all

    References for Anti-Granzyme B antibody [GB11] (ab10912)

    This product has been referenced in:
    • Demotte N  et al. A galectin-3 ligand corrects the impaired function of human CD4 and CD8 tumor-infiltrating lymphocytes and favors tumor rejection in mice. Cancer Res 70:7476-88 (2010). Flow Cyt ; Human . Read more (PubMed: 20719885) »

    See 1 Publication for this product

    Product Wall

    Thank you for contacting Abcam.

    The main difference between ab103157, Granzyme B antibody [CLB-GB11] (FITC) and ab10912, -Granzyme B antibody [ GB11 ] is that ab103157 is conjugated to a FITC tag, whereas ab10912 is unconjugated. Also althoug...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"