Anti-Granzyme B antibody - N-terminal, prediluted (ab15313)
- Datasheet
- Specific References (2)
- Protocols
Overview
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Product nameAnti-Granzyme B antibody - N-terminal, prediluted
See all Granzyme B primary antibodies -
DescriptionRabbit polyclonal to Granzyme B - N-terminal, prediluted
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Host speciesRabbit
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Tested applicationsSuitable for: ICC/IF, IHC-Pmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Dog, Pig
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Immunogen
Synthetic peptide corresponding to Human Granzyme B (N terminal).
Database link: P10144 -
Positive control
- IHC-P: Human tonsil tissue.
Properties
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FormPrediluted
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Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
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Storage bufferpH: 7.60
Preservative: 0.099% Sodium azide
Constituents: Tris buffered saline, 1% BSA -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Related Products
Applications
Our Abpromise guarantee covers the use of ab15313 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. PubMed: 18209028 | |
| IHC-P | 1/1. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Pre-diluted / ready to use |
Target
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FunctionThis enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.
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Sequence similaritiesBelongs to the peptidase S1 family. Granzyme subfamily.
Contains 1 peptidase S1 domain. -
Cellular localizationCytoplasmic granule. Cytoplasmic granules of cytolytic T-lymphocytes and natural killer cells.
- Information by UniProt
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Database links
- Entrez Gene: 490630 Dog
- Entrez Gene: 3002 Human
- Entrez Gene: 14939 Mouse
- Entrez Gene: 100233184 Pig
- Entrez Gene: 171528 Rat
- Omim: 123910 Human
- SwissProt: P10144 Human
- SwissProt: P04187 Mouse
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Alternative names
- C11 antibody
- Cathepsin G like 1 antibody
- Cathepsin G-like 1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody - N-terminal, prediluted (ab15313)Formalin-fixed, paraffin-embedded human tonsil tissue stained for Granzyme B using ab15313 in immunohistochemical analysis.
References
This product has been referenced in:
- Barcia C et al. In Vivo Polarization of IFN-{gamma} at Kupfer and Non-Kupfer Immunological Synapses during the Clearance of Virally Infected Brain Cells. J Immunol 180:1344-1352 (2008). ICC/IF ; Rat . Read more (PubMed: 18209028) »
- Zhang N et al. Targeted and untargeted CD137L fusion proteins for the immunotherapy of experimental solid tumors. Clin Cancer Res 13:2758-67 (2007). Read more (PubMed: 17460060) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"