Overview

  • Product nameGranzyme B (CTLA-1) Human ELISA Kit
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    A 15 356pg/ml 18 5.06%
    B 15 73pg/ml 5 7.38%
    A 15 356pg/ml 18 5.06%
    B 15 73pg/ml 5 7.38%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 20 pg/ml
  • Range
    31.25 pg/ml - 1000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 73 31.25pg/ml - 1000pg/ml

  • Assay time
    3h 45m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Granzyme B (CTLA-1) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Granzyme B in Human serum, plasma, buffered solutions and cell culture medium.

    A monoclonal antibody specific Granzyme B has been coated onto the wells of the microtiter strips provided. Samples, including standards of known Granzyme B concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for Granzyme B are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of Granzyme B present in the samples.

    This kit will recognize both endogenous and recombinant Human Granzyme B.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

  • RelevanceThe lytic enzymes of cytotoxic lymphocytes seem to be contained within lysosome-like granules found in the cytoplasm. These granules contain perforin, a membrane pore-forming protein, and a family of serine proteases (granzymes). Granzyme B, a 32 kDa protein, is a serine Aspase associated with the granules of Natural Killer (NK) cells. Granzyme B seems to be able to elicit cell death, perhaps by activating an endogenous pathway of programmed cell death.
  • Cellular localizationCytoplasmic
  • Alternative names
    • C11
    • Cathepsin G like 1
    • CTLA 1
    • CTSGL1
    • Cytotoxic T lymphocyte proteinase 2
    • EC 3.4.21.79
    • Fragmentin 2
    • Granzyme 2
    • HLP
    • Human lymphocyte protein
    • Lymphocyte protease
    • SECT
    • T cell serine protease 1 3E
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab46142 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Granzyme B (CTLA-1) Human ELISA Kit images

  •  Representative Standard Curve using ab46142

Protocols

References for Granzyme B (CTLA-1) Human ELISA Kit (ab46142)

This product has been referenced in:
  • Patel SA  et al. Mesenchymal stem cells protect breast cancer cells through regulatory T cells: role of mesenchymal stem cell-derived TGF-beta. J Immunol 184:5885-94 (2010). Human . Read more (PubMed: 20382885) »
  • Prakash MD  et al. Active and zymogen forms of granzyme B are constitutively released from cytotoxic lymphocytes in the absence of target cell engagement. Immunol Cell Biol 87:249-54 (2009). Human . Read more (PubMed: 19079360) »

See all 3 Publications for this product

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Antibodies included in the Granzyme kit are raised in mouse. The capture antibody coated onto the wells is (Fab)' 2 IgG1, and the biotiinylated detection antibody tracer is whole IgG1, and final concentration is ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"