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In Abcam's Granzyme B Inhibitor Screening Assay Kit, active human Granzyme B enzyme hydrolyzes the specific substrate to release the quench of fluorescent group, which can be detected fluorometrically at Ex/Em = 380/500 nm. In the presence of potent Granzyme B Inhibitor, the hydrolyzation of substrate will be impeded. The Kit provides a rapid, simple, sensitive and reliable test suitable for high throughput screening of Granzyme B inhibitors or characterize/study Granzyme B inhibitors. Inhibitor control, Ac-IEPD-CHO is included to compare the efficacy of test inhibitors.
Granzyme B (GZMB, EC number 220.127.116.11) also known as Granzyme-2 is a serine protease stored in the granules of activated cytotoxic T cells and NK cells. Upon target cell contact, Granzyme B is directionally exocytosed and with the assistance of perforin enters the target cell. With its unique substrate specificity (Granzyme B prefers an aspartic acid residue at the P1 site of its substrates), Granzyme B processes and activates various pro-caspases thereby inducing apoptosis in the target cell.
|Granzyme B Assay Buffer||WM||1 x 25ml|
|Granzyme B Enzyme||Green||1 vial|
|Granzyme B Substrate||Red||1 x 0.5ml|
|Inhibitor Control (Ac-IEPD-CHO)||Blue||1 x 20µl|
Our Abpromise guarantee covers the use of ab157405 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Inhibition Assay||Use at an assay dependent concentration.|
|Functional Studies||Use at an assay dependent concentration.|
ab157405 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"