Granzyme B overexpression 293T lysate (whole cell) (ab94040)

Overview

  • Product nameGranzyme B overexpression 293T lysate (whole cell)
    See all Granzyme B lysates
  • Description
    Granzyme B overexpression lysate
  • General notesab94040 is a 293T cell transfected lysate in which Human Granzyme B has been transiently over-expressed using a pCMV-Granzyme B plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
  • Tested applicationsSuitable for: WBmore details

Properties

  • BackgroundFunction: This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis. Similarity: Belongs to the peptidase S1 family. Granzyme subfamily. Contains 1 peptidase S1 domain.

Associated products

Applications

Our Abpromise guarantee covers the use of ab94040 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

Granzyme B overexpression 293T lysate (whole cell) images

  • ab94040 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-Granzyme B antibody (ab89415) at 1/500 dilution

    Lane 1 : Granzyme B 293T Transfected Lysate - Positive Control (ab94040)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References for Granzyme B overexpression 293T lysate (whole cell) (ab94040)

ab94040 has not yet been referenced specifically in any publications.

Product Wall

We determined overexpression of GZMB protein by Western Blot.

However, whether the expressed form is active or inactive has not been determined.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"