Granzyme B overexpression 293T lysate (whole cell) (ab94040)

Overview

  • Product name
    Granzyme B overexpression 293T lysate (whole cell)
    See all Granzyme B lysates
  • Description
    Granzyme B overexpression lysate
  • General notes
    ab94040 is a 293T cell transfected lysate in which Human Granzyme B has been transiently over-expressed using a pCMV-Granzyme B plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
  • Tested applications
    Suitable for: WBmore details

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 6.80
    Constituent: 100% 1x Sample Buffer
  • Concentration information loading...
  • Purity
    Whole Cell Lysate
  • Research areas
  • Background
    Function: This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis. Similarity: Belongs to the peptidase S1 family. Granzyme subfamily. Contains 1 peptidase S1 domain.

Associated products

Applications

Our Abpromise guarantee covers the use of ab94040 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

Images

  • ab94040 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-Granzyme B antibody (ab89415) at 1/500 dilution

    Lane 1 : Granzyme B 293T Transfected Lysate - Positive Control (ab94040)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References

ab94040 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

We determined overexpression of GZMB protein by Western Blot.

However, whether the expressed form is active or inactive has not been determined.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up