General notesab94041 is a 293T cell transfected lysate in which Human Granzyme B has been transiently over-expressed using a pCMV-Granzyme B plasmid. The lysate is provided in 1X Sample Buffer.
Note: For more detailed how the transfected lysate was prepared view preparation notes
BackgroundFunction: This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.
Similarity: Belongs to the peptidase S1 family. Granzyme subfamily.
Contains 1 peptidase S1 domain.