The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesICC/IF: 1/500 - 1/1000.
WB: 1/500 - 1/1000. Detects bands of approximately 21 and 54 kDa (predicted molecular weight: 27 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionCytotoxic chymotrypsin-like serine protease with preference for bulky and aromatic residues at the P1 position and acidic residues at the P3' and P4' sites. Probably necessary for target cell lysis in cell-mediated immune responses. Participates in the antiviral response via direct cleavage of several proteins essential for viral replication.
Tissue specificityConstituvely expressed in NK cells.
Sequence similaritiesBelongs to the peptidase S1 family. Granzyme subfamily. Contains 1 peptidase S1 domain.
Cellular localizationCytoplasmic granule. Cytoplasmic granules of cytolytic T-lymphocytes.
Immunocytochemistry/ Immunofluorescence - Anti-Granzyme H antibody (ab69876)
Immunofluorescence analysis of A549 cells using ab69876 at 1/500-1/1000 dilution. Left image un-treated.Right image treated with immunizing peptide.
References for Anti-Granzyme H antibody (ab69876)
This product has been referenced in:
Tang H et al. Granzyme H of cytotoxic lymphocytes is required for clearance of the hepatitis B virus through cleavage of the hepatitis B virus X protein. J Immunol188:824-31 (2012).
Read more (PubMed: 22156339) »