Overview

  • Product name
  • Description
    Rabbit polyclonal to Granzyme H
  • Specificity
    This antibody detects endogenous levels of total GZMH protein.
  • Tested applications
    Suitable for: WB, Indirect ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Granzyme H (internal sequence).

  • Positive control
    • A549 cells and extracts from K562 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab69876 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Indirect ELISA
ICC/IF
  • Application notes
    ICC/IF: 1/500 - 1/1000.
    I-ELISA: 1/5000.
    WB: 1/500 - 1/1000. Detects bands of approximately 21 and 54 kDa (predicted molecular weight: 27 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Cytotoxic chymotrypsin-like serine protease with preference for bulky and aromatic residues at the P1 position and acidic residues at the P3' and P4' sites. Probably necessary for target cell lysis in cell-mediated immune responses. Participates in the antiviral response via direct cleavage of several proteins essential for viral replication.
    • Tissue specificity
      Constituvely expressed in NK cells.
    • Sequence similarities
      Belongs to the peptidase S1 family. Granzyme subfamily.
      Contains 1 peptidase S1 domain.
    • Cellular localization
      Cytoplasmic granule. Cytoplasmic granules of cytolytic T-lymphocytes.
    • Information by UniProt
    • Database links
    • Alternative names
      • Cathepsin G like 2 antibody
      • Cathepsin G-like 2 antibody
      • CCP X antibody
      • CCP-X antibody
      • CGL 2 antibody
      • CGL2 antibody
      • CSP C antibody
      • CSP-C antibody
      • CTLA1 antibody
      • CTSGL2 antibody
      • Cytotoxic serine protease C antibody
      • Cytotoxic T lymphocyte associated serine esterase 1 antibody
      • Cytotoxic T lymphocyte proteinase antibody
      • Cytotoxic T-lymphocyte proteinase antibody
      • EC 3.4.21.- antibody
      • GRAH_HUMAN antibody
      • Granzyme H (cathepsin G-like 2, protein h-CCPX) antibody
      • Granzyme H antibody
      • GZMH antibody
      • Protein h CCPX antibody
      see all

    Images

    • All lanes : Anti-Granzyme H antibody (ab69876) at 1/500 dilution

      Lane 1 : Extracts from K562 cells
      Lane 2 : Extracts from K562 cells with immunizing peptide at 10 µg

      Lysates/proteins at 30 µg per lane.


      Predicted band size : 27 kDa
      Observed band size : 21 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 54 kDa. We are unsure as to the identity of these extra bands.
    • Immunofluorescence analysis of A549 cells using ab69876 at 1/500-1/1000 dilution.
      Left image un-treated.
      Right image treated with immunizing peptide.

    References

    This product has been referenced in:
    • Tang H  et al. Granzyme H of cytotoxic lymphocytes is required for clearance of the hepatitis B virus through cleavage of the hepatitis B virus X protein. J Immunol 188:824-31 (2012). WB, ICC/IF ; Human . Read more (PubMed: 22156339) »

    See 1 Publication for this product

    Customer reviews and Q&As

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Flow Cytometry
    Sample
    Human Cell (PBMC)
    Permeabilization
    Yes - Saponin
    Gating Strategy
    CD8 T cells
    Specification
    PBMC
    Preparation
    Cell harvesting/tissue preparation method: PBMCs were collected from blood using Ficoll
    Sample buffer: Cytometry staining Buffer: 2% FCS and 2mM EDTA and 0.025% azide in PBS
    Fixation
    Paraformaldehyde
    Username

    Daniel del Alcazar

    Verified customer

    Submitted Nov 06 2017

    Thank you for contacting us.

    We do not have this type of clinical data as our products are for in vitro use. A literature search should be able to provide you with this kind of information.


    I hope this information is helpful ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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