The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesWB: Use at a concentration of 1-5 µg/ml.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
FunctionCleaves peptide substrates after methionine, leucine, and norleucine. Physiological substrates include EZR, alpha-tubulins and the apoptosis inhibitor BIRC5/Survivin. Promotes caspase activation and subsequent apoptosis of target cells.
Tissue specificityHighly and constitutively expressed in activated natural killer (NK) cells.
Sequence similaritiesBelongs to the peptidase S1 family. Granzyme subfamily. Contains 1 peptidase S1 domain.
Cellular localizationSecreted. Cytoplasmic granule. Granules of large granular lymphocytes.