Overview

  • Product name
  • Description
    Rabbit polyclonal to GRAP
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 205-217 of Human GRAP (NP_006604).

  • Positive control
    • A549 whole cell lysate and U373 xenograft

Properties

Applications

Our Abpromise guarantee covers the use of ab97344 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 25 kDa.
IHC-P Use at an assay dependent concentration.

Target

Images

  • Anti-GRAP antibody (ab97344) at 1/1000 dilution + A549 whole cell lysate at 30 µg

    Predicted band size : 25 kDa
  • ab97344 at 1/100 dilution staining GRAP in paraffin-embedded U373 xenograft by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

References

ab97344 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for your reply and for sending the additional information.

What dilution of the primary had you tried? We recommend 1:1000, but you may want to try diluting more. I'd also recommend switching the blocking buffer to 5% milk throughou...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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