Overview

  • Product nameAnti-GRASP65 antibody [4F8]
    See all GRASP65 primary antibodies
  • Description
    Mouse monoclonal [4F8] to GRASP65
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length Human GRASP65 produced in HEK293T cells (NP_114105).

  • Positive control
    • HEK293T cell lysate transfected with pCMV6-ENTRY GRASP65 cDNA; COS7 cells transiently transfected by pCMV6-ENTRY GRASP65.
  • General notesStable for 12 months from date of receipt.

Properties

Applications

Our Abpromise guarantee covers the use of ab117932 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 46 kDa.
ICC/IF 1/100.

Target

  • FunctionStacking factor involved in the postmitotic assembly of Golgi stacks from mitotic Golgi fragments. Key structural protein required for the maintenance of the Golgi apparatus integrity: its caspase-mediated cleavage is required for fragmentation of the Golgi during apoptosis. Also mediates, via its interaction with GM130, the docking of transport vesicles with the Golgi membranes.
  • Sequence similaritiesBelongs to the GORASP family.
    Contains 1 PDZ (DHR) domain.
  • Post-translational
    modifications
    Phosphorylated by CDC2/B1 and PLK kinases during mitosis. Phosphorylation cycle correlates with the cisternal stacking cycle. Phosphorylation of the homodimer prevents the association of dimers into higher order oligomers, leading to cisternal unstacking.
    Target for caspase-3 cleavage during apoptosis. The cleavage contributes to Golgi fragmentation and occurs very early in the execution phase of apoptosis.
  • Cellular localizationGolgi apparatus > cis-Golgi network membrane. Undergoes rapid exchange with the cytosol.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ23443 antibody
    • Golgi peripheral membrane protein p65 antibody
    • Golgi phosphoprotein 5 antibody
    • Golgi reassembly and stacking protein 1 antibody
    • Golgi reassembly and stacking protein 65 kDa antibody
    • Golgi reassembly and stacking protein, 65-kD antibody
    • Golgi reassembly stacking protein 1 65kDa antibody
    • Golgi reassembly stacking protein 1 antibody
    • Golgi reassembly stacking protein of 65 kDa antibody
    • Golgi reassembly-stacking protein 1 antibody
    • Golgi reassembly-stacking protein of 65 kDa antibody
    • GOLPH 5 antibody
    • GOLPH5 antibody
    • Gorasp 1 antibody
    • GORASP1 antibody
    • GORS1_HUMAN antibody
    • GRASP 65 antibody
    • GRASP65 antibody
    • MGC118894 antibody
    • MGC118897 antibody
    • P65 antibody
    see all

Anti-GRASP65 antibody [4F8] images

  • ab117932 at 1/100 dilution staining GRASP65 in COS7 cells transiently transfected by pCMV6-ENTRY GRASP65 by Immunofluorescence.
  • All lanes : Anti-GRASP65 antibody [4F8] (ab117932) at 1/2000 dilution

    Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY GRASP65 cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 46 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC200828)

References for Anti-GRASP65 antibody [4F8] (ab117932)

ab117932 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"