Overview

  • Product nameAnti-GRASP65 antibody
    See all GRASP65 primary antibodies
  • Description
    Rabbit polyclonal to GRASP65
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within N-terminal amino acids 35-84 (PYFDFIITIG HSRLNKENDT LKALLKANVE KPVKLEVFNM KTMRVREVEV) of Human GRASP65 (NP_114105).

  • Positive control
    • OVCAR3 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab102645 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 46 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionStacking factor involved in the postmitotic assembly of Golgi stacks from mitotic Golgi fragments. Key structural protein required for the maintenance of the Golgi apparatus integrity: its caspase-mediated cleavage is required for fragmentation of the Golgi during apoptosis. Also mediates, via its interaction with GM130, the docking of transport vesicles with the Golgi membranes.
  • Sequence similaritiesBelongs to the GORASP family.
    Contains 1 PDZ (DHR) domain.
  • Post-translational
    modifications
    Phosphorylated by CDC2/B1 and PLK kinases during mitosis. Phosphorylation cycle correlates with the cisternal stacking cycle. Phosphorylation of the homodimer prevents the association of dimers into higher order oligomers, leading to cisternal unstacking.
    Target for caspase-3 cleavage during apoptosis. The cleavage contributes to Golgi fragmentation and occurs very early in the execution phase of apoptosis.
  • Cellular localizationGolgi apparatus > cis-Golgi network membrane. Undergoes rapid exchange with the cytosol.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ23443 antibody
    • Golgi peripheral membrane protein p65 antibody
    • Golgi phosphoprotein 5 antibody
    • Golgi reassembly and stacking protein 1 antibody
    • Golgi reassembly and stacking protein 65 kDa antibody
    • Golgi reassembly and stacking protein, 65-kD antibody
    • Golgi reassembly stacking protein 1 65kDa antibody
    • Golgi reassembly stacking protein 1 antibody
    • Golgi reassembly stacking protein of 65 kDa antibody
    • Golgi reassembly-stacking protein 1 antibody
    • Golgi reassembly-stacking protein of 65 kDa antibody
    • GOLPH 5 antibody
    • GOLPH5 antibody
    • Gorasp 1 antibody
    • GORASP1 antibody
    • GORS1_HUMAN antibody
    • GRASP 65 antibody
    • GRASP65 antibody
    • MGC118894 antibody
    • MGC118897 antibody
    • P65 antibody
    see all

Anti-GRASP65 antibody images

  • Anti-GRASP65 antibody (ab102645) at 1 µg/ml + OVCAR-3 cell lysate at 10 µg

    Predicted band size : 46 kDa

References for Anti-GRASP65 antibody (ab102645)

ab102645 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"