Overview

  • Product nameAnti-GRASP65 antibodySee all GRASP65 primary antibodies ...
  • Description
    Rabbit polyclonal to GRASP65
  • Tested applicationsIP, WB, ICC/IF, IHC-P, IHC-FoFr more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Xenopus laevis
  • Immunogen

    Recombinant full length 6-his tagged protein (Mouse)

Properties

Applications

Our Abpromise guarantee covers the use of ab30315 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IP Use at an assay dependent dilution.
WB 1/5000 - 1/10000. Predicted molecular weight: 46 kDa.
ICC/IF 1/5000.
IHC-P 1/5000.
IHC-FoFr Use at an assay dependent concentration. PubMed: 22355340

Target

  • FunctionStacking factor involved in the postmitotic assembly of Golgi stacks from mitotic Golgi fragments. Key structural protein required for the maintenance of the Golgi apparatus integrity: its caspase-mediated cleavage is required for fragmentation of the Golgi during apoptosis. Also mediates, via its interaction with GM130, the docking of transport vesicles with the Golgi membranes.
  • Sequence similaritiesBelongs to the GORASP family.
    Contains 1 PDZ (DHR) domain.
  • Post-translational
    modifications
    Phosphorylated by CDC2/B1 and PLK kinases during mitosis. Phosphorylation cycle correlates with the cisternal stacking cycle. Phosphorylation of the homodimer prevents the association of dimers into higher order oligomers, leading to cisternal unstacking.
    Target for caspase-3 cleavage during apoptosis. The cleavage contributes to Golgi fragmentation and occurs very early in the execution phase of apoptosis.
  • Cellular localizationGolgi apparatus > cis-Golgi network membrane. Undergoes rapid exchange with the cytosol.
  • Target information above from: UniProt accession Q9BQQ3 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • FLJ23443 antibody
    • Golgi peripheral membrane protein p65 antibody
    • Golgi phosphoprotein 5 antibody
    • Golgi reassembly and stacking protein 1 antibody
    • Golgi reassembly and stacking protein 65 kDa antibody
    • Golgi reassembly and stacking protein, 65-kD antibody
    • Golgi reassembly stacking protein 1 65kDa antibody
    • Golgi reassembly stacking protein 1 antibody
    • Golgi reassembly stacking protein of 65 kDa antibody
    • Golgi reassembly-stacking protein 1 antibody
    • Golgi reassembly-stacking protein of 65 kDa antibody
    • GOLPH 5 antibody
    • GOLPH5 antibody
    • Gorasp 1 antibody
    • Gorasp1 antibody
    • GORS1_HUMAN antibody
    • GRASP 65 antibody
    • GRASP65 antibody
    • MGC118894 antibody
    • MGC118897 antibody
    • P65 antibody
    see all

Anti-GRASP65 antibody images

  • GRASP65 antibody (ab30315) labelling in mouse Cor1 cell cells. Cor1 cells were fixed with formaldehyde and permeabilised. 1% BSA used as blocking agent for 10 mins. Primary antibody ab30315 incubated at rt°C at 1/5000 for 2 hours in TBS/BSA/azide/Triton.  Secondary Antibody Name: anti rabbit IgG Alexa Fluor® 594 1/1000. ab30315 in Cor1 cells appears to localise discretely to an intracellular compartment that could be the Golgi Appparatus. This pattern is very similar to that in this publication: http://jcb.rupress.org/cgi/content/full/156/3/411-a

    See Abreview

  • ab30315 staining GRASP65 in rat hippocampal tissue (sub-region) by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using citric acid pH 6.0. Samples were then blocked with 1% BSA for 10 minutes at room temperature followed by incubation with the primary antibody at a 1/6000 dilution for 16 hours. A biotin-conjugated anti-rabbit was used as secondary antibody at a 1/300 dilution. Nuclei are lightly counterstained with Haemalum.
  • ab30315, staining GRASP65 (green) in murine hippocampus tissue by Immunohistochemistry (PFA perfusion fixed frozen sections).

    Secions were incubated with primary antibody and an AlexaFluor®488-conjugated donkey anti-rabbit IgG was used as the secondary antibody.

References for Anti-GRASP65 antibody (ab30315)

This product has been referenced in:
  • Xia WF  et al. Vps35 loss promotes hyperresorptive osteoclastogenesis and osteoporosis via sustained RANKL signaling. J Cell Biol 200:821-37 (2013). Mouse . Read more (PubMed: 23509071) »
  • Matsuki T  et al. Identification of Stk25 as a genetic modifier of Tau phosphorylation in Dab1-mutant mice. PLoS One 7:e31152 (2012). IHC-FoFr ; Mouse . Read more (PubMed: 22355340) »

See all 6 Publications for this product

Product Wall

Thank you for providing that information.

As I mentioned previously, with the exception of ab79483, we do guarantee that these antibodies should work in western blot on rat tissue. If we cannot resolve the issue you are having with the antibod...

Read More

Thank you for contacting Abcam.

I am sorry that you are having some issues with our antibodies. All of the antibodies you mentioned should work in western blot in various species, however only ab30315 is known to work in IHC-P. Below is a list...

Read More
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Brain:hippocampal sub-region)
Specification Brain:hippocampal sub-region
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Apr 14 2009

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Brain:cerebellum)
Specification Brain:cerebellum
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Apr 08 2009

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Cor1 cells)
Specification Cor1 cells
Fixative Formaldehyde
Permeabilization Yes - 0,3% Triton added to my usual blocking buffer
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Apr 08 2009

Application Western blot
Sample Xenopus laevis Cell lysate - whole cell (egg extract)
Loading amount 50 µg
Specification egg extract
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Anne-Lore Schlaitz

Verified customer

Submitted Jun 30 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"