Anti-Graves disease carrier protein / GDC antibody (ab80499)

Overview

  • Product nameAnti-Graves disease carrier protein / GDC antibody
    See all Graves disease carrier protein / GDC primary antibodies
  • Description
    Rabbit polyclonal to Graves disease carrier protein / GDC
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Chimpanzee
  • Immunogen

    Synthetic peptide: VGLSHAPTLL GRPSSDNPNV LVLKTHVNLL CGGVAGAIAQ TISYPFDVTR , corresponding to internal sequence amino acids 215-264 of Human Graves disease carrier protein/ GDC

  • Positive control
    • 721_B cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab80499 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 36 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/62500.

Target

Anti-Graves disease carrier protein / GDC antibody images

  • Anti-Graves disease carrier protein / GDC antibody (ab80499) at 1 µg/ml + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 36 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 30 kDa,45 kDa,55 kDa. We are unsure as to the identity of these extra bands.

References for Anti-Graves disease carrier protein / GDC antibody (ab80499)

ab80499 has not yet been referenced specifically in any publications.

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