Anti-GRB10 antibody (ab154029)
Key features and details
- Rabbit polyclonal to GRB10
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GRB10 antibody
See all GRB10 primary antibodies -
Description
Rabbit polyclonal to GRB10 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 354-594 of Human GRB10 (UniProt: Q13322).
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Positive control
- WB: HepG2 whole cell lysate (ab7900).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154029 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
1/500 - 1/3000. Predicted molecular weight: 67 kDa.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 67 kDa. |
Target
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Function
Adapter protein which modulates coupling of a number of cell surface receptor kinases with specific signaling pathways. Binds to, and suppress signals from, activated receptors tyrosine kinases, including the insulin (INSR) and insulin-like growth factor (IGF1R) receptors. The inhibitory effect can be achieved by 2 mechanisms: interference with the signaling pathway and increased receptor degradation. Delays and reduces AKT1 phosphorylation in response to insulin stimulation. Blocks association between INSR and IRS1 and IRS2 and prevents insulin-stimulated IRS1 and IRS2 tyrosine phosphorylation. Recruits NEDD4 to IGF1R, leading to IGF1R ubiquitination, increased internalization and degradation by both the proteasomal and lysosomal pathways. May play a role in mediating insulin-stimulated ubiquitination of INSR, leading to proteasomal degradation. Negatively regulates Wnt signaling by interacting with LRP6 intracellular portion and interfering with the binding of AXIN1 to LRP6. Positive regulator of the KDR/VEGFR-2 signaling pathway. May inhibit NEDD4-mediated degradation of KDR/VEGFR-2. -
Tissue specificity
Widely expressed in fetal and adult tissues, including fetal and postnatal liver, lung, kidney, skeletal muscle, heart, spleen, skin and brain. -
Sequence similarities
Belongs to the GRB7/10/14 family.
Contains 1 PH domain.
Contains 1 Ras-associating domain.
Contains 1 SH2 domain. -
Domain
The PH domain binds relatively non-specifically to several phosphoinositides, including PI(5)P, PI(4,5)P2, PI(3,4)P2 and PI(3,4,5)P3, with modest affinities. -
Post-translational
modificationsphosphorylated on serine residues upon EGF, FGF and PDGF stimulation. -
Cellular localization
Cytoplasm. When complexed with NEDD4 and IGF1R, follows IGF1R internalization, remaining associated with early endosomes. Uncouples from IGF1R-containing endosomes before the sorting of the receptor to the lysosomal compartment. - Information by UniProt
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Database links
- Entrez Gene: 2887 Human
- Omim: 601523 Human
- SwissProt: Q13322 Human
- Unigene: 164060 Human
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Alternative names
- grb 10 antibody
- GRB IR antibody
- grb-10 antibody
see all
Images
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Anti-GRB10 antibody (ab154029) at 1/2000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 67 kDa7.5% SDS PAGE.
Secondary antibody - goat anti-rabbit HRP (ab6721)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab154029 has been referenced in 3 publications.
- Denhez B et al. Saturated fatty acids induce insulin resistance in podocytes through inhibition of IRS1 via activation of both IKKß and mTORC1. Sci Rep 10:21628 (2020). PubMed: 33303821
- Ge Q et al. Mulberry Leaf Regulates Differentially Expressed Genes in Diabetic Mice Liver Based on RNA-Seq Analysis. Front Physiol 9:1051 (2018). PubMed: 30131712
- García-Palmero I et al. The adaptors Grb10 and Grb14 are calmodulin-binding proteins. FEBS Lett 591:1176-1186 (2017). PubMed: 28295264