Overview

  • Product nameAnti-GRB10 antibody
    See all GRB10 primary antibodies
  • Description
    Mouse monoclonal to GRB10
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Recombinant Fragment
    Predicted to work with: Human
  • Immunogen

    Recombinant fragment: AVRRLQEEDQ QFRTSSLPAI PNPFPELCGP GSPPVLTPGS LPPSQAAAKQ DVKVFSEDGT SKVVEILADM TARDLCQLLV YKSHCVDDNS , corresponding to amino acids 61-151 of Human GRB 10

Properties

Applications

Our Abpromise guarantee covers the use of ab55153 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 1-5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionAdapter protein which modulates coupling of a number of cell surface receptor kinases with specific signaling pathways. Binds to, and suppress signals from, activated receptors tyrosine kinases, including the insulin (INSR) and insulin-like growth factor (IGF1R) receptors. The inhibitory effect can be achieved by 2 mechanisms: interference with the signaling pathway and increased receptor degradation. Delays and reduces AKT1 phosphorylation in response to insulin stimulation. Blocks association between INSR and IRS1 and IRS2 and prevents insulin-stimulated IRS1 and IRS2 tyrosine phosphorylation. Recruits NEDD4 to IGF1R, leading to IGF1R ubiquitination, increased internalization and degradation by both the proteasomal and lysosomal pathways. May play a role in mediating insulin-stimulated ubiquitination of INSR, leading to proteasomal degradation. Negatively regulates Wnt signaling by interacting with LRP6 intracellular portion and interfering with the binding of AXIN1 to LRP6. Positive regulator of the KDR/VEGFR-2 signaling pathway. May inhibit NEDD4-mediated degradation of KDR/VEGFR-2.
    • Tissue specificityWidely expressed in fetal and adult tissues, including fetal and postnatal liver, lung, kidney, skeletal muscle, heart, spleen, skin and brain.
    • Sequence similaritiesBelongs to the GRB7/10/14 family.
      Contains 1 PH domain.
      Contains 1 Ras-associating domain.
      Contains 1 SH2 domain.
    • DomainThe PH domain binds relatively non-specifically to several phosphoinositides, including PI(5)P, PI(4,5)P2, PI(3,4)P2 and PI(3,4,5)P3, with modest affinities.
    • Post-translational
      modifications
      phosphorylated on serine residues upon EGF, FGF and PDGF stimulation.
    • Cellular localizationCytoplasm. When complexed with NEDD4 and IGF1R, follows IGF1R internalization, remaining associated with early endosomes. Uncouples from IGF1R-containing endosomes before the sorting of the receptor to the lysosomal compartment.
    • Information by UniProt
    • Database links
    • Alternative names
      • grb 10 antibody
      • GRB IR antibody
      • grb-10 antibody
      • GRB10 adapter protein antibody
      • GRB10 adaptor protein antibody
      • GRB10 antibody
      • GRB10_HUMAN antibody
      • GRBIR antibody
      • Growth factor receptor bound protein 10 antibody
      • Growth factor receptor-bound protein 10 antibody
      • Insulin receptor binding protein antibody
      • Insulin receptor binding protein GRB IR antibody
      • Insulin receptor-binding protein Grb-IR antibody
      • IRBP antibody
      • KIAA0207 antibody
      • Maternally expressed gene 1 antibody
      • MEG1 antibody
      • RSS antibody
      see all

    Anti-GRB10 antibody images

    • Western blot against tagged recombinant protein immunogen using ab55153 GRB 10 antibody at 1ug/ml. Predicted band size of immunogen is 36 kDa.

      This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    References for Anti-GRB10 antibody (ab55153)

    ab55153 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"