• Product nameAnti-GRB10 antibody
    See all GRB10 primary antibodies
  • Description
    Rabbit polyclonal to GRB10
  • Specificityab61771 detects endogenous levels of total GRB 10 protein.
  • Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthesized non-phosphopeptide derived from human GRB 10 around the phosphorylation site of tyrosine 67 (S-L-YP-S-A).

  • Positive control
    • Human brain tissue; extracts from NIH/3T3 cells


Associated products


Our Abpromise guarantee covers the use of ab61771 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa).
IHC-P 1/50 - 1/100.
ELISA 1/5000.


  • FunctionAdapter protein which modulates coupling of a number of cell surface receptor kinases with specific signaling pathways. Binds to, and suppress signals from, activated receptors tyrosine kinases, including the insulin (INSR) and insulin-like growth factor (IGF1R) receptors. The inhibitory effect can be achieved by 2 mechanisms: interference with the signaling pathway and increased receptor degradation. Delays and reduces AKT1 phosphorylation in response to insulin stimulation. Blocks association between INSR and IRS1 and IRS2 and prevents insulin-stimulated IRS1 and IRS2 tyrosine phosphorylation. Recruits NEDD4 to IGF1R, leading to IGF1R ubiquitination, increased internalization and degradation by both the proteasomal and lysosomal pathways. May play a role in mediating insulin-stimulated ubiquitination of INSR, leading to proteasomal degradation. Negatively regulates Wnt signaling by interacting with LRP6 intracellular portion and interfering with the binding of AXIN1 to LRP6. Positive regulator of the KDR/VEGFR-2 signaling pathway. May inhibit NEDD4-mediated degradation of KDR/VEGFR-2.
  • Tissue specificityWidely expressed in fetal and adult tissues, including fetal and postnatal liver, lung, kidney, skeletal muscle, heart, spleen, skin and brain.
  • Sequence similaritiesBelongs to the GRB7/10/14 family.
    Contains 1 PH domain.
    Contains 1 Ras-associating domain.
    Contains 1 SH2 domain.
  • DomainThe PH domain binds relatively non-specifically to several phosphoinositides, including PI(5)P, PI(4,5)P2, PI(3,4)P2 and PI(3,4,5)P3, with modest affinities.
  • Post-translational
    phosphorylated on serine residues upon EGF, FGF and PDGF stimulation.
  • Cellular localizationCytoplasm. When complexed with NEDD4 and IGF1R, follows IGF1R internalization, remaining associated with early endosomes. Uncouples from IGF1R-containing endosomes before the sorting of the receptor to the lysosomal compartment.
  • Information by UniProt
  • Database links
  • Alternative names
    • grb 10 antibody
    • GRB IR antibody
    • grb-10 antibody
    • GRB10 adapter protein antibody
    • GRB10 adaptor protein antibody
    • GRB10 antibody
    • GRB10_HUMAN antibody
    • GRBIR antibody
    • Growth factor receptor bound protein 10 antibody
    • Growth factor receptor-bound protein 10 antibody
    • Insulin receptor binding protein antibody
    • Insulin receptor binding protein GRB IR antibody
    • Insulin receptor-binding protein Grb-IR antibody
    • IRBP antibody
    • KIAA0207 antibody
    • Maternally expressed gene 1 antibody
    • MEG1 antibody
    • RSS antibody
    see all

Anti-GRB10 antibody images

  • All lanes : Anti-GRB10 antibody (ab61771) at 1/500 dilution

    Lane 1 : extracts from NIH/3T3 cells, treated with Insulin (0.01U/ml, 15mins) with no peptide
    Lane 2 : extracts from NIH/3T3 cells, treated with Insulin (0.01U/ml, 15mins) with peptide

    Predicted band size : 67 kDa
    Observed band size : 30,40,67 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry analysis of paraffin-embedded human brain tissue using ab61771 at a dilution of 1/50. Left: sample untreated. Right: sample treated with immunising peptide.

References for Anti-GRB10 antibody (ab61771)

ab61771 has not yet been referenced specifically in any publications.

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