Overview

  • Product nameAnti-GRB2 antibody
    See all GRB2 primary antibodies
  • Description
    Goat polyclonal to GRB2
  • SpecificityExpected to recognize both reported isoforms (NP_002077.1 and NP_987102.1).
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Pig, Orangutan
  • Immunogen

    A KLH conjugated synthetic peptide corresponding to C-terminal amino acids 206-217(

    PRNYVTPVNRNV

    ) of Human GRB2 (P62993)

  • Positive control
    • Jurkat cell lysate Human pancreas tissue Human tonsil tissue (stains mantle zone and to a lessser extent germinal centre)

Properties

Applications

Our Abpromise guarantee covers the use of ab109651 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 25 kDa.
ELISA 1/32000.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Validated for use in over 20 tissue types.

Target

  • FunctionAdapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway.
    Isoform GRB3-3 does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Isoform GRB3-3 acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death.
  • Sequence similaritiesBelongs to the GRB2/sem-5/DRK family.
    Contains 1 SH2 domain.
    Contains 2 SH3 domains.
  • DomainThe SH3 domains mediate interaction with RALGPS1 and SHB.
  • Cellular localizationGolgi apparatus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Abundant SRC homology antibody
    • Adapter protein GRB2 antibody
    • ASH antibody
    • Ash protein antibody
    • EGFRBP GRB2 antibody
    • Epidermal growth factor receptor binding protein antibody
    • Epidermal growth factor receptor binding protein GRB2 antibody
    • GRB 2 antibody
    • GRB2 adapter protein antibody
    • Grb2 antibody
    • GRB2_HUMAN antibody
    • Grb3 3 antibody
    • Growth factor receptor bound protein 2 antibody
    • Growth factor receptor bound protein 3 antibody
    • Growth factor receptor-bound protein 2 antibody
    • HT027 antibody
    • MST084 antibody
    • MSTP084 antibody
    • NCKAP2 antibody
    • OTTHUMP00000166096 antibody
    • OTTHUMP00000166097 antibody
    • OTTHUMP00000166098 antibody
    • Protein ash antibody
    • SEM5 antibody
    • SH2/SH3 adapter GRB2 antibody
    see all

Anti-GRB2 antibody images

  • Anti-GRB2 antibody (ab109651) at 2 µg/ml + Jurkat lysate at 35 µg
    Developed using the ECL technique

    Predicted band size : 25 kDa


    Exposure time : 1 hour
  • ab109651, at 5µg/ml, staining Human GRB2 in pancreas, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.

References for Anti-GRB2 antibody (ab109651)

ab109651 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"