Overview

  • Product nameAnti-GRB2 antibody
    See all GRB2 primary antibodies
  • Description
    Rabbit polyclonal to GRB2
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Xenopus laevis, Zebrafish, Catfish
  • Immunogen

    Synthetic peptide, corresponding to C terminal amino acids 198-217 of Human GRB2

  • Positive control
    • Human Brain, Cerebellum, Human Brain, Cortex and Human Colon, Myenteric Plexus tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab111031 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 25 kDa.
IHC-P Use a concentration of 10 µg/ml.

Target

  • FunctionAdapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway.
    Isoform GRB3-3 does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Isoform GRB3-3 acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death.
  • Sequence similaritiesBelongs to the GRB2/sem-5/DRK family.
    Contains 1 SH2 domain.
    Contains 2 SH3 domains.
  • DomainThe SH3 domains mediate interaction with RALGPS1 and SHB.
  • Cellular localizationGolgi apparatus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Abundant SRC homology antibody
    • Adapter protein GRB2 antibody
    • ASH antibody
    • Ash protein antibody
    • EGFRBP GRB2 antibody
    • Epidermal growth factor receptor binding protein antibody
    • Epidermal growth factor receptor binding protein GRB2 antibody
    • GRB 2 antibody
    • GRB2 adapter protein antibody
    • Grb2 antibody
    • GRB2_HUMAN antibody
    • Grb3 3 antibody
    • Growth factor receptor bound protein 2 antibody
    • Growth factor receptor bound protein 3 antibody
    • Growth factor receptor-bound protein 2 antibody
    • HT027 antibody
    • MST084 antibody
    • MSTP084 antibody
    • NCKAP2 antibody
    • OTTHUMP00000166096 antibody
    • OTTHUMP00000166097 antibody
    • OTTHUMP00000166098 antibody
    • Protein ash antibody
    • SEM5 antibody
    • SH2/SH3 adapter GRB2 antibody
    see all

Anti-GRB2 antibody images

  • ab111031, at 10µg/ml, staining GRB2 in Formalin-fixed, Paraffin-embedded Human Brain cerebellum tissue by Immunohistochemistry.
  • ab111031, at 10µg/ml, staining GRB2 in Formalin-fixed, Paraffin-embedded Human Brain cortex tissue by Immunohistochemistry.
  • ab111031, at 10µg/ml, staining GRB2 in Formalin-fixed, Paraffin-embedded Human Colon Myenteric Plexus tissue by Immunohistochemistry.

References for Anti-GRB2 antibody (ab111031)

ab111031 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.

The following standard heat-mediated citrate buffer antigen retrieval was used for the IHC validation of ab32111 and ab111031. Antibody ab111586 used citrate buffer in a pressure cooker at 120C for 5 minutes but ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"