Overview

  • Product nameAnti-GRB2 antibody
    See all GRB2 primary antibodies
  • Description
    Goat polyclonal to GRB2
  • Tested applicationsSuitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Pig
    Predicted to work with: Cow, Dog
  • Immunogen

    Synthetic peptide: PRNYVTPVNRNV, corresponding to C terminal amino acids 206-217 of Human GRB2.

  • General notesPrincipal Names - GRB2; growth factor receptor-bound protein 2 Official Gene Symbol - GRB2. GenBank Accession Number – NP_002077. LocusLink ID - 2885. Gene Ontology terms - cell-cell signalling; SH3/SH2 adaptor protein; EGF receptor signalling pathway; RAS protein signal transduction; cell shape and cell size control.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, 0.5mg/ml Tris, pH 7.3
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab2234 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
IHC-P
WB
  • Application notesELISA: Use at an assay dependent dilution.
    IHC-P: Use at a concentration of 0.1 - 0.3 µg/ml. In paraffin embedded Human Tonsil, stains mantle zone and to a lessser extent germinal centre.
    WB: Use at a concentration of 1 - 3 µg/ml. Predicted molecular weight: 30 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionAdapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway.
      Isoform GRB3-3 does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Isoform GRB3-3 acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death.
    • Sequence similaritiesBelongs to the GRB2/sem-5/DRK family.
      Contains 1 SH2 domain.
      Contains 2 SH3 domains.
    • DomainThe SH3 domains mediate interaction with RALGPS1 and SHB.
    • Cellular localizationGolgi apparatus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Abundant SRC homology antibody
      • Adapter protein GRB2 antibody
      • ASH antibody
      • Ash protein antibody
      • EGFRBP GRB2 antibody
      • Epidermal growth factor receptor binding protein antibody
      • Epidermal growth factor receptor binding protein GRB2 antibody
      • GRB 2 antibody
      • GRB2 adapter protein antibody
      • Grb2 antibody
      • GRB2_HUMAN antibody
      • Grb3 3 antibody
      • Growth factor receptor bound protein 2 antibody
      • Growth factor receptor bound protein 3 antibody
      • Growth factor receptor-bound protein 2 antibody
      • HT027 antibody
      • MST084 antibody
      • MSTP084 antibody
      • NCKAP2 antibody
      • OTTHUMP00000166096 antibody
      • OTTHUMP00000166097 antibody
      • OTTHUMP00000166098 antibody
      • Protein ash antibody
      • SEM5 antibody
      • SH2/SH3 adapter GRB2 antibody
      see all

    Anti-GRB2 antibody images

    • All lanes : Anti-GRB2 antibody (ab2234) at 0.05 µg/ml

      Lane 1 : Mouse brain lysate
      Lane 2 : Rat brain lysate
      Lane 3 : Mouse spleen lysate
      Lane 4 : Rat spleen lysate

      Lysates/proteins at 35 µg per lane.


      Predicted band size : 30 kDa


    • Predicted band size : 30 kDa
    • Lane 1 : Anti-GRB2 antibody (ab2234) at 0.05 µg/ml
      Lane 2 : Anti-GRB2 antibody (ab2234) at 0.05 µg

      Lane 1 : Human thymus lysate
      Lane 2 : Molt4 cell lysate

      Lysates/proteins at 35 µg per lane.


      Predicted band size : 30 kDa
    • Anti-GRB2 antibody (ab2234) at 2 µg/ml + Jurkat lysate (RIPA buffer, 35µg total protein per lane).

      Predicted band size : 30 kDa
      Observed band size : 30 kDa
      Ab2234 staining (2µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
    • Ab2234 (0.3ug/ml) staining of paraffin embedded Human Tonsil. Microwaved antigen retrieval with citrate buffer pH6, HRP-staining.

    References for Anti-GRB2 antibody (ab2234)

    ab2234 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (U2OS cells)
    Loading amount 50 µg
    Specification U2OS cells
    Gel Running Conditions Reduced Denaturing (6-15%)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted May 18 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"