Overview

  • Product name
    Anti-GRB2 antibody [GRB-232]
    See all GRB2 primary antibodies
  • Description
    Mouse monoclonal [GRB-232] to GRB2
  • Specificity
    Reacts specifically with an epitope in the C-terminal region of Grb2 (a.a. 200-217).
  • Tested applications
    Suitable for: IP, WB, ELISA, IHC-Pmore details
    Unsuitable for: ICC/IF
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Cow, Xenopus laevis, Zebrafish, Cynomolgus monkey
  • Immunogen

    Synthetic peptide:

    GQTGMFPRNYVTPVNRNV

    conjugated to KLH, corresponding to C terminal amino acids 200-217 of Human GRB2

  • Positive control
    • Whole extract of cultured human acute T cell leukemia Jurkat cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 15mM Sodium Azide
    Constituents: 0.01M PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    Purified from culture supernatant of hybridoma cells.
  • Clonality
    Monoclonal
  • Clone number
    GRB-232
  • Isotype
    IgG3
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab49876 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 25 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Function
      Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway.
      Isoform GRB3-3 does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Isoform GRB3-3 acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death.
    • Sequence similarities
      Belongs to the GRB2/sem-5/DRK family.
      Contains 1 SH2 domain.
      Contains 2 SH3 domains.
    • Domain
      The SH3 domains mediate interaction with RALGPS1 and SHB.
    • Cellular localization
      Golgi apparatus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Abundant SRC homology antibody
      • Adapter protein GRB2 antibody
      • ASH antibody
      • Ash protein antibody
      • EGFRBP GRB2 antibody
      • Epidermal growth factor receptor binding protein antibody
      • Epidermal growth factor receptor binding protein GRB2 antibody
      • GRB 2 antibody
      • GRB2 adapter protein antibody
      • Grb2 antibody
      • GRB2_HUMAN antibody
      • Grb3 3 antibody
      • Growth factor receptor bound protein 2 antibody
      • Growth factor receptor bound protein 3 antibody
      • Growth factor receptor-bound protein 2 antibody
      • HT027 antibody
      • MST084 antibody
      • MSTP084 antibody
      • NCKAP2 antibody
      • OTTHUMP00000166096 antibody
      • OTTHUMP00000166097 antibody
      • OTTHUMP00000166098 antibody
      • Protein ash antibody
      • SEM5 antibody
      • SH2/SH3 adapter GRB2 antibody
      see all

    References

    This product has been referenced in:
    • Fritsche L  et al. Insulin-induced serine phosphorylation of IRS-2 via ERK1/2 and mTOR: studies on the function of Ser675 and Ser907. Am J Physiol Endocrinol Metab 300:E824-36 (2011). IP ; Human . Read more (PubMed: 21098738) »
    • Chentouf M  et al. Excessive food intake, obesity and inflammation process in Zucker fa/fa rat pancreatic islets. PLoS One 6:e22954 (2011). WB ; Rat . Read more (PubMed: 21826222) »

    See all 2 Publications for this product

    Customer reviews and Q&As

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (mouse D3 embryonic stem cells)
    Permeabilization
    Yes - triton 0.1%
    Specification
    mouse D3 embryonic stem cells
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 15°C
    Fixative
    Paraformaldehyde
    Username

    Miss. Fabiana Luise

    Verified customer

    Submitted Nov 30 2015

    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (mouse embryonic stem cells D3)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    1e+007 cells
    Specification
    mouse embryonic stem cells D3
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    Submitted Nov 18 2015

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - other (Hela cell subcellular fractionation)
    Loading amount
    10 µg
    Specification
    Hela cell subcellular fractionation
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Dec 17 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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