Overview

  • Product name
    Anti-GRB2 antibody [M331]
    See all GRB2 primary antibodies
  • Description
    Mouse monoclonal [M331] to GRB2
  • Tested applications
    Suitable for: WB, IP, ELISAmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant full length Rat protein

  • Positive control
    • PC12 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab86713 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 24 kDa.
IP 1/50.
ELISA 1/2000.

Target

  • Function
    Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway.
    Isoform GRB3-3 does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Isoform GRB3-3 acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death.
  • Sequence similarities
    Belongs to the GRB2/sem-5/DRK family.
    Contains 1 SH2 domain.
    Contains 2 SH3 domains.
  • Domain
    The SH3 domains mediate interaction with RALGPS1 and SHB.
  • Cellular localization
    Golgi apparatus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Abundant SRC homology antibody
    • Adapter protein GRB2 antibody
    • ASH antibody
    • Ash protein antibody
    • EGFRBP GRB2 antibody
    • Epidermal growth factor receptor binding protein antibody
    • Epidermal growth factor receptor binding protein GRB2 antibody
    • GRB 2 antibody
    • GRB2 adapter protein antibody
    • Grb2 antibody
    • GRB2_HUMAN antibody
    • Grb3 3 antibody
    • Growth factor receptor bound protein 2 antibody
    • Growth factor receptor bound protein 3 antibody
    • Growth factor receptor-bound protein 2 antibody
    • HT027 antibody
    • MST084 antibody
    • MSTP084 antibody
    • NCKAP2 antibody
    • OTTHUMP00000166096 antibody
    • OTTHUMP00000166097 antibody
    • OTTHUMP00000166098 antibody
    • Protein ash antibody
    • SEM5 antibody
    • SH2/SH3 adapter GRB2 antibody
    see all

Images

  • Lane 1 : Anti-GRB2 antibody [M331] (ab86713) at 1/250 dilution
    Lane 2 : Anti-GRB2 antibody [M331] (ab86713) at 1/500 dilution
    Lane 3 : Anti-GRB2 antibody [M331] (ab86713) at 1/1000 dilution

    Lane 1 : PC12 cell lysate
    Lane 2 : PC12 cell lysate
    Lane 3 : PC12 cell lysate


    Observed band size : 24 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:
  • Spangle JM & Munger K The HPV16 E6 oncoprotein causes prolonged receptor protein tyrosine kinase signaling and enhances internalization of phosphorylated receptor species. PLoS Pathog 9:e1003237 (2013). WB ; Human . Read more (PubMed: 23516367) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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