The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Detects a band of approximately 24 kDa.
FunctionAdapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway. Isoform GRB3-3 does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Isoform GRB3-3 acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death.
Sequence similaritiesBelongs to the GRB2/sem-5/DRK family. Contains 1 SH2 domain. Contains 2 SH3 domains.
DomainThe SH3 domains mediate interaction with RALGPS1 and SHB.
References for Anti-GRB2 antibody [M331] (ab86713)
This product has been referenced in:
Spangle JM & Munger K The HPV16 E6 oncoprotein causes prolonged receptor protein tyrosine kinase signaling and enhances internalization of phosphorylated receptor species. PLoS Pathog9:e1003237 (2013).
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