Overview

  • Product nameAnti-GRB2 antibody [Y237]
    See all GRB2 primary antibodies
  • Description
    Rabbit monoclonal [Y237] to GRB2
  • Tested applicationsSuitable for: WB, IP, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in the C terminus of human GRB2

  • Positive control
    • 293 cell lysate, human ovarian carcinoma, HeLa cells.
  • General notes

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab32037 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/10000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
IP 1/50.
IHC-P 1/100 - 1/250.
ICC/IF 1/50 - 1/100.
  • Application notesIs unsuitable for Flow Cyt.
  • Target

    • FunctionAdapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway.
      Isoform GRB3-3 does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Isoform GRB3-3 acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death.
    • Sequence similaritiesBelongs to the GRB2/sem-5/DRK family.
      Contains 1 SH2 domain.
      Contains 2 SH3 domains.
    • DomainThe SH3 domains mediate interaction with RALGPS1 and SHB.
    • Cellular localizationGolgi apparatus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Abundant SRC homology antibody
      • Adapter protein GRB2 antibody
      • ASH antibody
      • Ash protein antibody
      • EGFRBP GRB2 antibody
      • Epidermal growth factor receptor binding protein antibody
      • Epidermal growth factor receptor binding protein GRB2 antibody
      • GRB 2 antibody
      • GRB2 adapter protein antibody
      • Grb2 antibody
      • GRB2_HUMAN antibody
      • Grb3 3 antibody
      • Growth factor receptor bound protein 2 antibody
      • Growth factor receptor bound protein 3 antibody
      • Growth factor receptor-bound protein 2 antibody
      • HT027 antibody
      • MST084 antibody
      • MSTP084 antibody
      • NCKAP2 antibody
      • OTTHUMP00000166096 antibody
      • OTTHUMP00000166097 antibody
      • OTTHUMP00000166098 antibody
      • Protein ash antibody
      • SEM5 antibody
      • SH2/SH3 adapter GRB2 antibody
      see all

    Anti-GRB2 antibody [Y237] images

    • All lanes : Anti-GRB2 antibody [Y237] (ab32037) at 1/10000 dilution

      Lane 1 : PC-12(Rat adrenal gland pheochromocytoma cell line)
      Lane 2 : NIH/3T3(Mouse embryonic fibroblast cell line)
      Lane 3 : HEK 293(Human epithelial cell line from embryonic kidney)

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/2000 dilution

      Predicted band size : 25 kDa
      Observed band size : 25 kDa

      Blocking/Dilution buffer : 5% NFDM/TBST

      Exposure time : 3 minutes

    • Immunohistochemical analysis of GRB2 expression in paraffin embedded human ovarian carcinoma, using 1/100 ab32037.
    • Immunofluorescent analysis of GRB2 expression in HeLa cells using 1/50 ab32037.
    • Anti-GRB2 antibody [Y237] (ab32037) at 1/5000 dilution + Lysate prepared from human Mesothelial cell line MeT-5A at 4 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 25 kDa
      Observed band size : 24 kDa (why is the actual band size different from the predicted?)


      Exposure time : 1 minute

      This image is a courtesy of Anonymous Abreview

      See Abreview

    • Anti-GRB2 antibody [Y237] (ab32037) at 1/5000 dilution + Recombinant human AHA1 protein (ab80352) at 0.01 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 25 kDa


      Exposure time : 2 minutes

    References for Anti-GRB2 antibody [Y237] (ab32037)

    This product has been referenced in:
    • Li K  et al. Genome-Wide Transcriptional Analysis Reveals the Protection against Hypoxia-Induced Oxidative Injury in the Intestine of Tibetans via the Inhibition of GRB2/EGFR/PTPN11 Pathways. Oxid Med Cell Longev 2016:6967396 (2016). WB ; Human . Read more (PubMed: 27594973) »
    • Chang W  et al. Gene expression profiling-derived immunohistochemistry signature with high prognostic value in colorectal carcinoma. Gut N/A:N/A (2013). WB ; Human . Read more (PubMed: 24173294) »

    See all 3 Publications for this product

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Mouse Cell (D3)
    Permeabilization Yes - 0.1% Triton
    Specification D3
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1%
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Oct 22 2015

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (Mesothelial cell line MeT-5A (ATCC CRL-9444))
    Loading amount 4 µg
    Specification Mesothelial cell line MeT-5A (ATCC CRL-9444)
    Gel Running Conditions Reduced Denaturing (GE Healthcare, Excelgel 12.5 homogenous)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Sep 11 2009

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (Jurkat)
    Loading amount 500000 cells
    Specification Jurkat
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted Dec 03 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"