Overview

  • Product name
    Anti-GRB2 antibody [Y237]
    See all GRB2 primary antibodies
  • Description
    Rabbit monoclonal [Y237] to GRB2
  • Tested applications
    Suitable for: Flow Cyt, WB, IP, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human GRB2 aa 200 to the C-terminus (C terminal). The exact sequence is proprietary.

  • Positive control
    • WB: HEK293, PC-12 and NIH/3T3 cell lysates. IHC-P: Human ovarian carcinoma tissue. ICC/IF: HeLa cells. Flow Cyt: HeLa cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab32037 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/20.

ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB 1/5000 - 1/10000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
IP 1/50.
IHC-P 1/100 - 1/250.
ICC/IF 1/50 - 1/100.

Target

  • Function
    Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway.
    Isoform GRB3-3 does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Isoform GRB3-3 acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death.
  • Sequence similarities
    Belongs to the GRB2/sem-5/DRK family.
    Contains 1 SH2 domain.
    Contains 2 SH3 domains.
  • Domain
    The SH3 domains mediate interaction with RALGPS1 and SHB.
  • Cellular localization
    Golgi apparatus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Abundant SRC homology antibody
    • Adapter protein GRB2 antibody
    • ASH antibody
    • Ash protein antibody
    • EGFRBP GRB2 antibody
    • Epidermal growth factor receptor binding protein antibody
    • Epidermal growth factor receptor binding protein GRB2 antibody
    • GRB 2 antibody
    • GRB2 adapter protein antibody
    • Grb2 antibody
    • GRB2_HUMAN antibody
    • Grb3 3 antibody
    • Growth factor receptor bound protein 2 antibody
    • Growth factor receptor bound protein 3 antibody
    • Growth factor receptor-bound protein 2 antibody
    • HT027 antibody
    • MST084 antibody
    • MSTP084 antibody
    • NCKAP2 antibody
    • OTTHUMP00000166096 antibody
    • OTTHUMP00000166097 antibody
    • OTTHUMP00000166098 antibody
    • Protein ash antibody
    • SEM5 antibody
    • SH2/SH3 adapter GRB2 antibody
    see all

Anti-GRB2 antibody [Y237] images

  • All lanes : Anti-GRB2 antibody [Y237] (ab32037) at 1/10000 dilution

    Lane 1 : PC-12 (rat adrenal gland pheochromocytoma) cell lysate
    Lane 2 : NIH/3T3 (mouse embryonic fibroblast) cell lysate
    Lane 3 : HEK293 (human epithelial cell line from embryonic kidney) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/2000 dilution

    Predicted band size : 25 kDa
    Observed band size : 25 kDa

    Blocking/Dilution buffer : 5% NFDM/TBST

    Exposure time : 3 minutes

  • Immunohistochemical analysis of GRB2 expression in paraffin embedded human ovarian carcinoma, using 1/100 ab32037.
  • Immunofluorescent analysis of GRB2 expression in HeLa cells using 1/50 ab32037.
  • Flow cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling GRB2 (red) with ab32037 at a 1/20 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Anti-GRB2 antibody [Y237] (ab32037) at 1/5000 dilution + Lysate prepared from human Mesothelial cell line MeT-5A at 4 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 25 kDa
    Observed band size : 24 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    This image is a courtesy of Anonymous Abreview

    See Abreview

  • Anti-GRB2 antibody [Y237] (ab32037) at 1/5000 dilution + Recombinant human AHA1 protein (ab80352) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 25 kDa


    Exposure time : 2 minutes

References for Anti-GRB2 antibody [Y237] (ab32037)

This product has been referenced in:

See all 5 Publications for this product

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (D3)
Permeabilization
Yes - 0.1% Triton
Specification
D3
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1%
Fixative
Paraformaldehyde
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Abcam user community

Verified customer

Submitted Oct 22 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Mesothelial cell line MeT-5A (ATCC CRL-9444))
Loading amount
4 µg
Specification
Mesothelial cell line MeT-5A (ATCC CRL-9444)
Gel Running Conditions
Reduced Denaturing (GE Healthcare, Excelgel 12.5 homogenous)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Sep 11 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Jurkat)
Loading amount
500000 cells
Specification
Jurkat
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Dec 03 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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