The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/5000 - 1/10000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
1/100 - 1/250.
1/50 - 1/100.
Application notesIs unsuitable for Flow Cyt.
FunctionAdapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway. Isoform GRB3-3 does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Isoform GRB3-3 acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death.
Sequence similaritiesBelongs to the GRB2/sem-5/DRK family. Contains 1 SH2 domain. Contains 2 SH3 domains.
DomainThe SH3 domains mediate interaction with RALGPS1 and SHB.
Western blot - Anti-GRB2 antibody [Y237] (ab32037)
Anti-GRB2 antibody [Y237] (ab32037) at 1/5000 dilution + Recombinant human AHA1 protein (ab80352) at 0.01 µg
Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 25 kDa
Exposure time : 2 minutes
References for Anti-GRB2 antibody [Y237] (ab32037)
This product has been referenced in:
Li K et al. Genome-Wide Transcriptional Analysis Reveals the Protection against Hypoxia-Induced Oxidative Injury in the Intestine of Tibetans via the Inhibition of GRB2/EGFR/PTPN11 Pathways. Oxid Med Cell Longev2016:6967396 (2016).
Read more (PubMed: 27594973) »
Chang W et al. Gene expression profiling-derived immunohistochemistry signature with high prognostic value in colorectal carcinoma. GutN/A:N/A (2013).
Read more (PubMed: 24173294) »