Anti-GRB2 antibody [Y301] (ab32111)


  • Product nameAnti-GRB2 antibody [Y301]
    See all GRB2 primary antibodies
  • Description
    Rabbit monoclonal [Y301] to GRB2
  • Specificityab32111 recognises GRB2. This antibody can also detect the splice isoform GRB3-3 of GRB2.
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in the N terminus of human GRB2

  • Positive control
    • Human breast carcinoma, NIH3T3 cells, PC12 cells, HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.


    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.



Our Abpromise guarantee covers the use of ab32111 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Predicted molecular weight: 25 kDa.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/40.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notesIs unsuitable for IP.
  • Target

    • FunctionAdapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway.
      Isoform GRB3-3 does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Isoform GRB3-3 acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death.
    • Sequence similaritiesBelongs to the GRB2/sem-5/DRK family.
      Contains 1 SH2 domain.
      Contains 2 SH3 domains.
    • DomainThe SH3 domains mediate interaction with RALGPS1 and SHB.
    • Cellular localizationGolgi apparatus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Abundant SRC homology antibody
      • Adapter protein GRB2 antibody
      • ASH antibody
      • Ash protein antibody
      • EGFRBP GRB2 antibody
      • Epidermal growth factor receptor binding protein antibody
      • Epidermal growth factor receptor binding protein GRB2 antibody
      • GRB 2 antibody
      • GRB2 adapter protein antibody
      • Grb2 antibody
      • GRB2_HUMAN antibody
      • Grb3 3 antibody
      • Growth factor receptor bound protein 2 antibody
      • Growth factor receptor bound protein 3 antibody
      • Growth factor receptor-bound protein 2 antibody
      • HT027 antibody
      • MST084 antibody
      • MSTP084 antibody
      • NCKAP2 antibody
      • OTTHUMP00000166096 antibody
      • OTTHUMP00000166097 antibody
      • OTTHUMP00000166098 antibody
      • Protein ash antibody
      • SEM5 antibody
      • SH2/SH3 adapter GRB2 antibody
      see all

    Anti-GRB2 antibody [Y301] images

    • All lanes : Anti-GRB2 antibody [Y301] (ab32111) at 1/2000 dilution

      Lane 1 : NIH 3T3 cell lysate
      Lane 2 : PC12 cell lysate
      Lane 3 : HeLa cell lysate

      Predicted band size : 25 kDa
      Observed band size : 25 kDa
    • Immunohistochemical analysis of GRB2 expression in paraffin embedded human breast carcinoma tissue section, using 1/100 ab32111.
    • Immunofluorescent analysis of GRB2 expression in PC12 cells, using 1/100 ab32111.
    • Overlay histogram showing SH-SY5Y cells stained with ab32111 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32111, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    References for Anti-GRB2 antibody [Y301] (ab32111)

    ab32111 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting us.

    The following standard heat-mediated citrate buffer antigen retrieval was used for the IHC validation of ab32111 and ab111031. Antibody ab111586 used citrate buffer in a pressure cooker at 120C for 5 minutes but ...

    Read More

    We used a heat mediated antigen retrieval method with sodium citrate, pH. 6.0.

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (Raji)
    Loading amount 40 µg
    Specification Raji
    Gel Running Conditions Reduced Denaturing (12%)
    Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

    Abcam user community

    Verified customer

    Submitted Sep 11 2009