Anti-GRB7 antibody [EPR4378] (ab109618)

Overview

  • Product nameAnti-GRB7 antibody [EPR4378]
    See all GRB7 primary antibodies
  • Description
    Rabbit monoclonal [EPR4378] to GRB7
  • Tested applicationsSuitable for: WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human GRB7.

  • Positive control
    • SKBR-3 and HeLa cell lysates. Human breast tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20ºC.
  • Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • PurityTissue culture supernatant
  • ClonalityMonoclonal
  • Clone numberEPR4378
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab109618 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 60 kDa.
IHC-P 1/50 - 1/100. (Heat to 98°C, allow to cool for 10-20 minutes)
ICC 1/50 - 1/100.
  • Application notesIs unsuitable for Flow Cyt or IP.
  • Target

    • FunctionInteracts with the cytoplasmic domain of the epidermal growth factor receptor which is then inhibited. The interaction is mediated by the SH2 domain. Also binds to ERBB2.
    • Sequence similaritiesBelongs to the GRB7/10/14 family.
      Contains 1 PH domain.
      Contains 1 Ras-associating domain.
      Contains 1 SH2 domain.
    • Post-translational
      modifications
      Phosphorylated on tyrosine residues.
    • Information by UniProt
    • Database links
    • Alternative names
      • B47 antibody
      • Epidermal growth factor receptor GRB 7 antibody
      • Epidermal growth factor receptor GRB-7 antibody
      • GRB7 adapter protein antibody
      • GRB7 antibody
      • GRB7_HUMAN antibody
      • Growth factor receptor bound protein 7 antibody
      • Growth factor receptor-bound protein 7 antibody
      • OTTHUMP00000164349 antibody
      • OTTHUMP00000164350 antibody
      • OTTHUMP00000164351 antibody
      • OTTHUMP00000164352 antibody
      see all

    Anti-GRB7 antibody [EPR4378] images

    • All lanes : Anti-GRB7 antibody [EPR4378] (ab109618) at 1/1000 dilution

      Lane 1 : SKBR-3 cell lysate
      Lane 2 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-labelled Goat anti-Rabbit at 1/2000 dilution

      Predicted band size : 60 kDa
    • ab109618 at 1/50 dilution staining GRB7 in paraffin-embedded Human breast tissue by Immunohistochemistry.

    References for Anti-GRB7 antibody [EPR4378] (ab109618)

    ab109618 has not yet been referenced specifically in any publications.

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    The concentration of lot GR48723-8 is 4.252 mg/ml.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"