• Product nameAnti-GRC5 / PHF2 antibody
    See all GRC5 / PHF2 primary antibodies
  • Description
    Rabbit polyclonal to GRC5 / PHF2
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 650-700 of Human GRC5/ PHF2 using the numbering given in entry NP_005383.3

  • Positive control
    • HeLa and 293T whole cell cell lysates



Our Abpromise guarantee covers the use of ab123507 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 120 kDa.
  • Application notesIs unsuitable for IP.
  • Target

    • FunctionLysine demethylase that demethylates both histones and non-histone proteins. Enzymatically inactive by itself, and becomes active following phosphorylation by PKA: forms a complex with ARID5B and mediates demethylation of methylated ARID5B. Demethylation of ARID5B leads to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes. The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver. PHF2 is recruited to trimethylated 'Lys-4' of histone H3 (H3K4me3) at rDNA promoters and promotes expression of rDNA.
    • Tissue specificityWidely expressed, including in liver (at protein level).
    • Sequence similaritiesBelongs to the JHDM1 histone demethylase family. JHDM1D subfamily.
      Contains 1 JmjC domain.
      Contains 1 PHD-type zinc finger.
    • DomainThe PHD-type zinc finger mediates the binding to H3K4me2 and H3K4me3.
    • Post-translational
      Phosphorylated by PKA on specific serine residues, leading to the formation of an active lysine demethylase complex.
    • Cellular localizationNucleus > nucleolus.
    • Information by UniProt
    • Database links
    • Alternative names
      • CENP-35 antibody
      • GRC5 antibody
      • JHDM1E antibody
      • Jumonji C domain containing histone demethylase 1E antibody
      • Lysine-specific demethylase PHF2 antibody
      • PHD finger protein 2 antibody
      • Phf2 antibody
      • PHF2_HUMAN antibody
      see all

    Anti-GRC5 / PHF2 antibody images

    • All lanes : Anti-GRC5 / PHF2 antibody (ab123507) at 0.1 µg/ml

      Lane 1 : Whole cell lysate from HeLa at 15 µg
      Lane 2 : Whole cell lysate from HeLa at 50 µg
      Lane 3 : Whole cell lysate from 293T at 50 µg
      Lane 4 : Whole cell lysate from Jurkat at 50 µg

      developed using the ECL technique

      Predicted band size : 120 kDa

      Exposure time : 30 seconds

    References for Anti-GRC5 / PHF2 antibody (ab123507)

    ab123507 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab123507.
    Please use the links above to contact us or submit feedback about this product.