Overview

  • Product name
  • Description
    Rabbit polyclonal to GRC5 / PHF2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 650-700 of Human GRC5/ PHF2 using the numbering given in entry NP_005383.3

  • Positive control
    • HeLa and 293T whole cell cell lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab123507 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 120 kDa.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Lysine demethylase that demethylates both histones and non-histone proteins. Enzymatically inactive by itself, and becomes active following phosphorylation by PKA: forms a complex with ARID5B and mediates demethylation of methylated ARID5B. Demethylation of ARID5B leads to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes. The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver. PHF2 is recruited to trimethylated 'Lys-4' of histone H3 (H3K4me3) at rDNA promoters and promotes expression of rDNA.
    • Tissue specificity
      Widely expressed, including in liver (at protein level).
    • Sequence similarities
      Belongs to the JHDM1 histone demethylase family. JHDM1D subfamily.
      Contains 1 JmjC domain.
      Contains 1 PHD-type zinc finger.
    • Domain
      The PHD-type zinc finger mediates the binding to H3K4me2 and H3K4me3.
    • Post-translational
      modifications
      Phosphorylated by PKA on specific serine residues, leading to the formation of an active lysine demethylase complex.
    • Cellular localization
      Nucleus > nucleolus.
    • Information by UniProt
    • Database links
    • Alternative names
      • CENP-35 antibody
      • GRC5 antibody
      • JHDM1E antibody
      • Jumonji C domain containing histone demethylase 1E antibody
      • Lysine-specific demethylase PHF2 antibody
      • PHD finger protein 2 antibody
      • Phf2 antibody
      • PHF2_HUMAN antibody
      see all

    Images

    • All lanes : Anti-GRC5 / PHF2 antibody (ab123507) at 0.1 µg/ml

      Lane 1 : Whole cell lysate from HeLa at 15 µg
      Lane 2 : Whole cell lysate from HeLa at 50 µg
      Lane 3 : Whole cell lysate from 293T at 50 µg
      Lane 4 : Whole cell lysate from Jurkat at 50 µg

      Developed using the ECL technique.

      Predicted band size: 120 kDa


      Exposure time: 30 seconds

    References

    ab123507 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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