Anti-GRC5 / PHF2 antibody (ab65771)
Key features and details
- Rabbit polyclonal to GRC5 / PHF2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GRC5 / PHF2 antibody
See all GRC5 / PHF2 primary antibodies -
Description
Rabbit polyclonal to GRC5 / PHF2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide, corresponding to C terminal amino acids 830-1096 of Human GRC5/ PHF2
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Positive control
- Nuclear extracts of HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Constituent: Whole serum -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab65771 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 130 kDa (predicted molecular weight: 121 kDa).
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Notes |
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WB
1/1000. Detects a band of approximately 130 kDa (predicted molecular weight: 121 kDa). |
Target
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Function
Lysine demethylase that demethylates both histones and non-histone proteins. Enzymatically inactive by itself, and becomes active following phosphorylation by PKA: forms a complex with ARID5B and mediates demethylation of methylated ARID5B. Demethylation of ARID5B leads to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes. The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver. PHF2 is recruited to trimethylated 'Lys-4' of histone H3 (H3K4me3) at rDNA promoters and promotes expression of rDNA. -
Tissue specificity
Widely expressed, including in liver (at protein level). -
Sequence similarities
Belongs to the JHDM1 histone demethylase family. JHDM1D subfamily.
Contains 1 JmjC domain.
Contains 1 PHD-type zinc finger. -
Domain
The PHD-type zinc finger mediates the binding to H3K4me2 and H3K4me3. -
Post-translational
modificationsPhosphorylated by PKA on specific serine residues, leading to the formation of an active lysine demethylase complex. -
Cellular localization
Nucleus > nucleolus. - Information by UniProt
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Database links
- Entrez Gene: 5253 Human
- Omim: 604351 Human
- SwissProt: O75151 Human
- Unigene: 211441 Human
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Alternative names
- CENP-35 antibody
- GRC5 antibody
- JHDM1E antibody
see all
Images
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Anti-GRC5 / PHF2 antibody (ab65771) at 1/1000 dilution + Nuclear extracts of HeLa cells.
Predicted band size: 121 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
Datasheets and documents
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Datasheet download
References (0)
ab65771 has not yet been referenced specifically in any publications.