Anti-GRC5 / PHF2 antibody - Carboxyterminal end (ab65771)

Overview

  • Product nameAnti-GRC5 / PHF2 antibody - Carboxyterminal end
    See all GRC5 / PHF2 primary antibodies
  • Description
    Rabbit polyclonal to GRC5 / PHF2 - Carboxyterminal end
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to C terminal amino acids 830-1096 of Human GRC5/ PHF2

  • Positive control
    • Nuclear extracts of HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab65771 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 130 kDa (predicted molecular weight: 121 kDa).
IP Use at an assay dependent dilution.

Target

  • FunctionLysine demethylase that demethylates both histones and non-histone proteins. Enzymatically inactive by itself, and becomes active following phosphorylation by PKA: forms a complex with ARID5B and mediates demethylation of methylated ARID5B. Demethylation of ARID5B leads to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes. The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver. PHF2 is recruited to trimethylated 'Lys-4' of histone H3 (H3K4me3) at rDNA promoters and promotes expression of rDNA.
  • Tissue specificityWidely expressed, including in liver (at protein level).
  • Sequence similaritiesBelongs to the JHDM1 histone demethylase family. JHDM1D subfamily.
    Contains 1 JmjC domain.
    Contains 1 PHD-type zinc finger.
  • DomainThe PHD-type zinc finger mediates the binding to H3K4me2 and H3K4me3.
  • Post-translational
    modifications
    Phosphorylated by PKA on specific serine residues, leading to the formation of an active lysine demethylase complex.
  • Cellular localizationNucleus > nucleolus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CENP-35 antibody
    • GRC5 antibody
    • JHDM1E antibody
    • Jumonji C domain containing histone demethylase 1E antibody
    • Lysine-specific demethylase PHF2 antibody
    • PHD finger protein 2 antibody
    • Phf2 antibody
    • PHF2_HUMAN antibody
    see all

Anti-GRC5 / PHF2 antibody - Carboxyterminal end images

  • Anti-GRC5 / PHF2 antibody - Carboxyterminal end (ab65771) at 1/1000 dilution + Nuclear extracts of HeLa cells.

    Predicted band size : 121 kDa
    Observed band size : 130 kDa (why is the actual band size different from the predicted?)

References for Anti-GRC5 / PHF2 antibody - Carboxyterminal end (ab65771)

ab65771 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us and for your patience while I have been in touch with the lab regarding your enquiry.
You are correct that there was a mistake on the datasheet. The immunogen runs from amino acid 830 to the C-terminus at amino acid 109...

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