Overview

  • Product nameAnti-GREB1 antibody
    See all GREB1 primary antibodies
  • Description
    Rabbit polyclonal to GREB1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide within residues: DNGHNALLGF SGNCVGCGKK GFCYFTEFSN HINLKLTTQP KKQKHLKYYL , corresponding to internal sequence amino acids 144-193 of Human GREB1(NP_683701)

  • Positive control
    • MCF7 cell lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab84025 is purified by a peptide affinity chromatography method.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab84025 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionUnknown. Primary target for estrogen receptor regulation. Acts as a regulator of hormone-dependent cancer growth in breast and prostate cancers.
  • Tissue specificityExpressed in proliferating prostatic tissue and prostate cancer.
  • Sequence similaritiesBelongs to the GREB1 family.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5730583K22Rik antibody
    • 9130004E13 antibody
    • AF180470 antibody
    • AU023194 antibody
    • Gene regulated by estrogen in breast cancer protein antibody
    • Gene regulated in breast cancer 1 protein antibody
    • GREB 1 antibody
    • Greb1 antibody
    • GREB1_HUMAN antibody
    • KIAA0575 antibody
    • mKIAA0575 antibody
    • OTTHUMP00000180581 antibody
    • Protein GREB1 antibody
    see all

Anti-GREB1 antibody images

  • Anti-GREB1 antibody (ab84025) at 1 µg/ml + MCF7 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 43 kDa
    Observed band size : 43 kDa

References for Anti-GREB1 antibody (ab84025)

ab84025 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Tissue lysate - whole (brain)
Total protein in input 800 µg
Specification brain
Immuno-precipitation step Protein A/G
Username

Abcam user community

Verified customer

Submitted Dec 25 2009

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (brain)
Specification brain
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: sodium citrate
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Jing-ping Zhang

Verified customer

Submitted Dec 18 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"