Overview

  • Product name
    Anti-GRF2 antibody
    See all GRF2 primary antibodies
  • Description
    Rabbit polyclonal to GRF2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Dog, Chimpanzee, Rhesus monkey, Orangutan, Bat
  • Immunogen

    Synthetic peptide corresponding to a region between residues 50 and 100 of human GRF2 (NP_005303.2)

  • Positive control
    • HeLa whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab86224 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notes
    IP: Use at 2 - 5 µg/mg of lysate.
    WB: 1/2000 - 1/10,000. Predicted molecular weight: 121 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Function
      Guanine nucleotide-releasing protein that binds to SH3 domain of CRK and GRB2/ASH. Transduces signals from CRK to activate RAS.
    • Tissue specificity
      Ubiquitously expressed in adult and fetus. Expression is high in adult skeletal muscle and placenta and in fetal brain and heart. Low levels of expression in adult and fetal liver.
    • Sequence similarities
      Contains 1 N-terminal Ras-GEF domain.
      Contains 1 Ras-GEF domain.
    • Information by UniProt
    • Database links
      • Form
        It localizes to cytoplasm and endosomes.
      • Alternative names
        • C3G antibody
        • C3G protein antibody
        • CRK SH3 binding GNRP antibody
        • CRK SH3-binding GNRP antibody
        • DKFZp781P1719 antibody
        • GRF 2 antibody
        • GRF2 antibody
        • Guanine nucleotide releasing factor 2 (specific for crk proto oncogene) antibody
        • Guanine nucleotide releasing factor 2 antibody
        • Guanine nucleotide-releasing factor 2 antibody
        • OTTHUMP00000064558 antibody
        • Protein C3G antibody
        • Rap guanine nucleotide exchange factor (GEF) 1 antibody
        • Rap guanine nucleotide exchange factor 1 antibody
        • RAPGEF 1 antibody
        • RAPGEF1 antibody
        • RPGF1_HUMAN antibody
        see all

      Images

      • Western blot - Anti-GRF2 antibody (ab86224)
        Western blot - Anti-GRF2 antibody (ab86224)
        All lanes : Anti-GRF2 antibody (ab86224) at 0.04 µg/ml

        Lane 1 : HeLa whole cell lysate at 50 µg
        Lane 2 : HeLa whole cell lysate at 15 µg
        Lane 3 : HeLa whole cell lysate at 5 µg

        Developed using the ECL technique.

        Predicted band size: 121 kDa


        Exposure time: 3 minutes
      • Immunoprecipitation - Anti-GRF2 antibody (ab86224)
        Immunoprecipitation - Anti-GRF2 antibody (ab86224)
        Detection of human GRF2 in Immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab86224 at 3 µg/mg lysate for IP, and at 1 µg/ml for subsequent Western blot.

        Detection: Chemiluminescence with an exposure time of 10 seconds.

      References

      ab86224 has not yet been referenced specifically in any publications.

      Publishing research using ab86224? Please let us know so that we can cite the reference in this datasheet.

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