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Rabbit polyclonal to GRF2 (phospho Y504)
ab74068 detects endogenous levels of GRF2 only when phosphorylated at tyrosine 504 (Human: Tyr504; Mouse: Tyr514).
Predicted to work with:
Synthetic phosphopeptide derived from human GRF2 around the phosphorylation site of tyrosine 504 (V-P-Y
Extracts from HepG2 cells treated with Na
3VO 4 (0.3nM, 40mins).
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg 2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
Immunogen affinity purified
ab74068 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 121 kDa (predicted molecular weight: 121 kDa).
Guanine nucleotide-releasing protein that binds to SH3 domain of CRK and GRB2/ASH. Transduces signals from CRK to activate RAS.
Ubiquitously expressed in adult and fetus. Expression is high in adult skeletal muscle and placenta and in fetal brain and heart. Low levels of expression in adult and fetal liver.
Contains 1 N-terminal Ras-GEF domain.
Contains 1 Ras-GEF domain.
Information by UniProt
It localizes to cytoplasm and endosomes.
C3G protein antibody
CRK SH3 binding GNRP antibody
Western blot - GRF2 (phospho Y504) antibody (ab74068)
All lanes : Anti-GRF2 (phospho Y504) antibody (ab74068) at 1/500 dilution Lane 1 : Extracts from HepG2 cells
treated with Na 3VO 4 (0.3nM, 40mins), Lane 2 : Extracts from HepG2 cells
treated with Na 3VO 4 (0.3nM, 40mins) with immunising phosphopeptide at 10 µg Lysates/proteins at 30 µg per lane. Predicted band size : 121 kDa Observed band size : 121 kDa
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"