Anti-GRF2 (phospho Y504) antibody (ab74068)

Overview

  • Product nameAnti-GRF2 (phospho Y504) antibody
    See all GRF2 primary antibodies
  • Description
    Rabbit polyclonal to GRF2 (phospho Y504)
  • Specificityab74068 detects endogenous levels of GRF2 only when phosphorylated at tyrosine 504 (Human: Tyr504; Mouse: Tyr514).
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic phosphopeptide derived from human GRF2 around the phosphorylation site of tyrosine 504 (V-P-YP-A-P)

  • Positive control
    • Extracts from HepG2 cells treated with Na3VO4 (0.3nM, 40mins).

Properties

Applications

Our Abpromise guarantee covers the use of ab74068 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 121 kDa (predicted molecular weight: 121 kDa).
ELISA 1/10000.

Target

  • FunctionGuanine nucleotide-releasing protein that binds to SH3 domain of CRK and GRB2/ASH. Transduces signals from CRK to activate RAS.
  • Tissue specificityUbiquitously expressed in adult and fetus. Expression is high in adult skeletal muscle and placenta and in fetal brain and heart. Low levels of expression in adult and fetal liver.
  • Sequence similaritiesContains 1 N-terminal Ras-GEF domain.
    Contains 1 Ras-GEF domain.
  • Information by UniProt
  • Database links
  • FormIt localizes to cytoplasm and endosomes.
  • Alternative names
    • C3G antibody
    • C3G protein antibody
    • CRK SH3 binding GNRP antibody
    • CRK SH3-binding GNRP antibody
    • DKFZp781P1719 antibody
    • GRF 2 antibody
    • GRF2 antibody
    • Guanine nucleotide releasing factor 2 (specific for crk proto oncogene) antibody
    • Guanine nucleotide releasing factor 2 antibody
    • Guanine nucleotide-releasing factor 2 antibody
    • OTTHUMP00000064558 antibody
    • Protein C3G antibody
    • Rap guanine nucleotide exchange factor (GEF) 1 antibody
    • Rap guanine nucleotide exchange factor 1 antibody
    • RAPGEF 1 antibody
    • RAPGEF1 antibody
    • RPGF1_HUMAN antibody
    see all

Anti-GRF2 (phospho Y504) antibody images

  • All lanes : Anti-GRF2 (phospho Y504) antibody (ab74068) at 1/500 dilution

    Lane 1 : Extracts from HepG2 cells treated with Na3VO4 (0.3nM, 40mins),
    Lane 2 : Extracts from HepG2 cells treated with Na3VO4 (0.3nM, 40mins) with immunising phosphopeptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 121 kDa
    Observed band size : 121 kDa

References for Anti-GRF2 (phospho Y504) antibody (ab74068)

ab74068 has not yet been referenced specifically in any publications.

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