Overview

  • Product name
  • Description
    Rabbit polyclonal to GRHL3
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    A region within synthetic peptide: SGVKGCLLSG FRGNETTYLR PETDLETPPV LFIPNVHFSS LQRSGGAAPS, corresponding to amino acids 428-477 of Human GRHL3

  • Positive control
    • HepG2 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab50755 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 70 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/12500.

Target

  • Function
    May function as a transcription factor.
  • Tissue specificity
    Expressed in brain, colon, pancreas, placenta and kidney. Isoform 1 is expressed in lung and tonsil. Isoform 2 is prostate-specific.
  • Sequence similarities
    Belongs to the grh/CP2 family. Grainyhead subfamily.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Drosophila antibody
    • Grainyhead like 3 antibody
    • Grainyhead like protein 3 homolog antibody
    • Grainyhead-like protein 3 homolog antibody
    • grhl3 antibody
    • GRHL3_HUMAN antibody
    • Sister of mammalian grainyhead antibody
    • Sister of mammalian grainyhead protein antibody
    • SOM antibody
    • TFCP2L4 antibody
    • Transcription factor CP2 like 4 antibody
    • Transcription factor CP2-like 4 antibody
    see all

Images

  • Lane 1 : marker
    Lane 2 : Anti-GRHL3 antibody (ab50755) at 0.5 µg/ml

    Lane 1 : As above
    Lane 2 : HepG2 cell lysate at 10 µg

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50,000.


    Predicted band size : 70 kDa
    Observed band size : ~57 kDa (why is the actual band size different from the predicted?)

References

ab50755 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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