Anti-GRHPR antibody - C-terminal (ab176011)

Overview

  • Product name
    Anti-GRHPR antibody - C-terminal
    See all GRHPR primary antibodies
  • Description
    Rabbit polyclonal to GRHPR - C-terminal
  • Tested applications
    Suitable for: ICC/IF, Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human GRHPR aa 299-328 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q9UBQ7

  • Positive control
    • Mouse kidney lysate, Human hepatocarcinoma tissue, HL60 or MCF-7 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab176011 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/10 - 1/50.
Flow Cyt 1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 36 kDa.

Target

  • Function
    Enzyme with hydroxy-pyruvate reductase, glyoxylate reductase and D-glycerate dehydrogenase enzymatic activities. Reduces hydroxypyruvate to D-glycerate, glyoxylate to glycolate oxidizes D-glycerate to hydroxypyruvate.
  • Tissue specificity
    Ubiquitous. Most abundantly expressed in the liver.
  • Involvement in disease
    Defects in GRHPR are the cause of hyperoxaluria primary type 2 (HP2) [MIM:260000]; also known as primary hyperoxaluria type II (PH2). HP2 is a disorder where the main clinical manifestation is calcium oxalate nephrolithiasis though chronic as well as terminal renal insufficiency has been described. It is characterized by an elevated urinary excretion of oxalate and L-glycerate.
  • Sequence similarities
    Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.
  • Information by UniProt
  • Database links
  • Alternative names
    • GLXR antibody
    • glycerate 2 dehydrogenase antibody
    • GLYD antibody
    • Glyoxylate reductase/hydroxypyruvate reductase antibody
    • Grhpr antibody
    • GRHPR_HUMAN antibody
    • OTTHUMP00000021379 antibody
    • OTTHUMP00000021380 antibody
    • OTTHUMP00000046131 antibody
    • PH 2 antibody
    • PH2 antibody
    • Primary hyperoxaluria type 2 antibody
    see all

Images

  • Anti-GRHPR antibody - C-terminal (ab176011) at 1/1000 dilution + Mouse kidney tissue lysate at 35 µg

    Predicted band size : 36 kDa
  • Immunofluorescence analysis of MCF-7 cells labeling GRHPR with ab176011 at a 1/10 dilution, followed by Alexa Fluor 488 conjugated goat anti-rabbit lgG. DAPI was used to stain the cell nuclear (blue).

  • Flow cytometric analysis of HL60 cells (green) compared to a negative control cell (blue) using ab176011 at a 1/10 dilution. FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • Immunohistochemical analysis of formalin fixed, paraffin embedded  Human hepatocarcinoma tissue labeling GRHPR with ab176011 at a 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

References

ab176011 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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