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Synthetic peptide within Human GRHPR aa 299-328 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
Database link: Q9UBQ7
Our Abpromise guarantee covers the use of ab176011 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/10 - 1/50.|
|Flow Cyt||1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/1000. Predicted molecular weight: 36 kDa.|
Immunofluorescence analysis of MCF-7 cells labeling GRHPR with ab176011 at a 1/10 dilution, followed by Alexa Fluor 488 conjugated goat anti-rabbit lgG. DAPI was used to stain the cell nuclear (blue).
Flow cytometric analysis of HL60 cells (green) compared to a negative control cell (blue) using ab176011 at a 1/10 dilution. FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Immunohistochemical analysis of formalin fixed, paraffin embedded Human hepatocarcinoma tissue labeling GRHPR with ab176011 at a 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.
ab176011 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"