Anti-GluK1 antibody (ab67316)
Key features and details
- Rabbit polyclonal to GluK1
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-GluK1 antibody
See all GluK1 primary antibodies -
Description
Rabbit polyclonal to GluK1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide corresponding to Human GluK1 (C terminal).
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Positive control
- P815 cells. Brain tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab67316 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 20 µg/ml.
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IHC-P |
Use at an assay dependent concentration.
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WB |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 104 kDa (predicted molecular weight: 104 kDa).
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Notes |
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ICC/IF
Use a concentration of 20 µg/ml. |
IHC-P
Use at an assay dependent concentration. |
WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 104 kDa (predicted molecular weight: 104 kDa). |
Target
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Function
Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. May be involved in the transmission of light information from the retina to the hypothalamus. -
Tissue specificity
Most abundant in the cerebellum and the suprachiasmatic nuclei (SCN) of the hypothalamus. -
Sequence similarities
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIK1 subfamily. -
Cellular localization
Cell membrane. Cell junction > synapse > postsynaptic cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 14805 Mouse
- SwissProt: Q60934 Mouse
- Unigene: 5134 Mouse
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Alternative names
- EAA3 antibody
- EEA3 antibody
- Excitatory amino acid receptor 3 antibody
see all
Images
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Immunofluorescence of GluK1 in Mouse Brain cells using ab67316 at 20 ug/ml.
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Paraffin-embedded mouse brain tissue stained for GluK1 using ab67316 at 5 µg/ml in immunohistochemical analysis.
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Lane 1 : Anti-GluK1 antibody (ab67316) at 0.5 µg/ml
Lane 2 : Anti-GluK1 antibody (ab67316) at 1 µg/ml
Lane 3 : Anti-GluK1 antibody (ab67316) at 2 µg/ml
All lanes : P815 cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 104 kDa
Observed band size: 104 kDa
Additional bands at: 95 kDa. We are unsure as to the identity of these extra bands.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab67316 has been referenced in 1 publication.
- Domínguez-Sala E et al. Properties of the epileptiform activity in the cingulate cortex of a mouse model of LIS1 dysfunction. Brain Struct Funct 227:1599-1614 (2022). PubMed: 35103859