Overview

  • Product nameAnti-GRIK2 antibody [EPR6307]
    See all GRIK2 primary antibodies
  • Description
    Rabbit monoclonal [EPR6307] to GRIK2
  • Tested applicationsSuitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to residues in the extracellular domain of Human GRIK2.

  • Positive control
    • Human fetal brain, Human kidney, and A431 lysates; Human kidney, brain and colon tissues.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab124702 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 103 kDa (predicted molecular weight: 103 kDa).Can be blocked with GRIK2 peptide (ab169992).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notesIs unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • FunctionIonotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. May be involved in the transmission of light information from the retina to the hypothalamus. Modulates cell surface expression of NETO2.
    • Tissue specificityExpression is higher in cerebellum than in cerebral cortex.
    • Involvement in diseaseDefects in GRIK2 are the cause of mental retardation autosomal recessive type 6 (MRT6) [MIM:611092]. It is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. In contrast to syndromic or specific mental retardation which also present with associated physical, neurological and/or psychiatric manifestations, intellectual deficiency is the only primary symptom of non-syndromic mental retardation. MRT6 patients display mild to severe mental retardation and psychomotor development delay in early childhood. Patients do not have neurologic problems, congenital malformations, or facial dysmorphism. Body height, weight, and head circumference are normal.
    • Sequence similaritiesBelongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIK2 subfamily.
    • Post-translational
      modifications
      Sumoylation mediates kainate receptor-mediated endocytosis and regulates synaptic transmission. Sumoylation is enhanced by PIAS3 and desumoylated by SENP1.
      Ubiquitinated. Ubiquitination regulates the GRIK2 levels at the synapse by leading kainate receptor degradation through proteasome.
    • Cellular localizationCell membrane. Cell junction > synapse > postsynaptic cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • bA487F5.1 antibody
      • EAA4 antibody
      • Excitatory amino acid receptor 4 antibody
      • GLR 6 antibody
      • GLR6 antibody
      • GluK2 antibody
      • GLUK6 antibody
      • GLUR 6 antibody
      • GluR-6 antibody
      • GluR6 antibody
      • Glutamate receptor 6 antibody
      • Glutamate receptor antibody
      • glutamate receptor form A antibody
      • glutamate receptor form B antibody
      • glutamate receptor form C antibody
      • glutamate receptor form D antibody
      • glutamate receptor form E antibody
      • Glutamate receptor ionotropic kainate 2 antibody
      • GRIK 2 antibody
      • GRIK2 antibody
      • GRIK2 protein antibody
      • GRIK2_HUMAN antibody
      • ionotropic kainate 2 antibody
      • MRT6 antibody
      see all

    Anti-GRIK2 antibody [EPR6307] images

    • All lanes : Anti-GRIK2 antibody [EPR6307] (ab124702) at 1/1000 dilution

      Lane 1 : Human fetal brain lysate
      Lane 2 : Human kidney lysate
      Lane 3 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 103 kDa
    • ab124702, at 1/250 dilution, staining GRIK2 in paraffin-embedded Human brain tissue by immunohistochemistry.
    • ab124702, at 1/250 dilution, staining GRIK2 in paraffin-embedded Human colon tissue by immunohistochemistry.
    • ab124702, at 1/250 dilution, staining GRIK2 in paraffin-embedded Human kidney tissue by immunohistochemistry.

    References for Anti-GRIK2 antibody [EPR6307] (ab124702)

    This product has been referenced in:

    See 1 Publication for this product

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    This peptide has been added to the catalog as ab169992 and is now available for order for $166.


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