• Product nameAnti-GRIM19 antibody [6E1BH7]
    See all GRIM19 primary antibodies
  • Description
    Mouse monoclonal [6E1BH7] to GRIM19
  • Tested applicationsSuitable for: IHC-P, WB, ICC/IF, ELISA, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
  • Immunogen

    Recombinant full length protein Human GRIM19.

  • Positive control
    • Human heart, Bovine heart, Rat heart, and Mouse heart isolated mitochondria, Human fibroblasts, HeLa cells
  • General notes

    Product was previously marketed under the MitoSciences sub-brand.




Our Abpromise guarantee covers the use of ab110240 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 17 kDa.
ICC/IF Use a concentration of 1 µg/ml.
ELISA Use a concentration of 8 µg/ml. In-Cell ELISA (0.8 µg/well)
Flow Cyt Use a concentration of 1 µg/ml. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.


  • FunctionAccessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Involved in the interferon/all-trans-retinoic acid (IFN/RA) induced cell death. This apoptotic activity is inhibited by interaction with viral IRF1. Prevents the transactivation of STAT3 target genes. May play a role in CARD15-mediated innate mucosal responses and serve to regulate intestinal epithelial cell responses to microbes.
  • Tissue specificityWidely expressed, with highest expression in heart, skeletal muscle, liver, kidney and placenta. In intestinal mucosa, down-regulated in areas involved in Crohn disease and ulcerative colitis.
  • Involvement in diseaseDefects in NDUFA13 may be a cause of susceptibility to Hurthle cell thyroid carcinoma (HCTC) [MIM:607464]. Hurthle cell thyroid carcinoma accounts for approximately 3% of all thyroid cancers. Although they are classified as variants of follicular neoplasms, they are more often multifocal and somewhat more aggressive and are less likely to take up iodine than are other follicular neoplasms.
  • Sequence similaritiesBelongs to the complex I NDUFA13 subunit family.
  • Developmental stageExpressed in numerous fetal tissues.
  • Cellular localizationMitochondrion inner membrane. Nucleus. May be translocated into the nucleus upon IFN/RA treatment.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2700054G14Rik antibody
    • AU022060 antibody
    • B16.6 antibody
    • CDA016 antibody
    • Cell death regulatory protein antibody
    • Cell death regulatory protein GRIM-19 antibody
    • CGI-39 antibody
    • CGI39 protein antibody
    • CI-B16.6 antibody
    • Complex I-B16.6 antibody
    • FLJ58045 antibody
    • FLJ59191 antibody
    • Gene associated with retinoic and IFN-induced mortality 19 protein antibody
    • Gene associated with retinoic and interferon-induced mortality 19 protein antibody
    • Gene associated with retinoic interferon induced mortality 19 protein antibody
    • GRIM-19 antibody
    • NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 13 antibody
    • NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 antibody
    • NADH ubiquinone oxidoreductase B16.6 subunit antibody
    • NADH-ubiquinone oxidoreductase B16.6 subunit antibody
    • NDUAD_HUMAN antibody
    • NDUFA13 antibody
    • RGD1565358 antibody
    see all

Anti-GRIM19 antibody [6E1BH7] images

  • All lanes : Anti-GRIM19 antibody [6E1BH7] (ab110240) at 1 µg/ml

    Lane 1 : Human heart mitochondria
    Lane 2 : Bovine heart mitochondria
    Lane 3 : Rat heart mitochondria
    Lane 4 : Mouse heart mitochondria

    Predicted band size : 17 kDa
  • Mitochondrial localization of GRIM19 visualized by immunocytochemistry using ab110240 at a concentration of 1 µg/mL. Cultured Human fibroblasts were fixed, permeabilized and then labeled with ab110240 followed by Alexa® 488 goat-anti-mouse IgG.
  • HeLa cells were stained with 1 µg/mL ab110240 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
  • ab110240 staining GRIM19 in Human cerebellum tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

    Tissue was fixed with formaldehyde and antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/3000 in TBS) for 1 hour and stained with DAB.

    See Abreview

References for Anti-GRIM19 antibody [6E1BH7] (ab110240)

This product has been referenced in:
  • Yue X  et al. GRIM-19 inhibition induced autophagy through activation of ERK and HIF-1a not STAT3 in Hela cells. Tumour Biol 37:9789-96 (2016). Read more (PubMed: 26810068) »
  • Guarani V  et al. TIMMDC1/C3orf1 functions as a membrane-embedded mitochondrial complex I assembly factor through association with the MCIA complex. Mol Cell Biol 34:847-61 (2014). Read more (PubMed: 24344204) »

See all 17 Publications for this product

Product Wall

Thank you for contacting us.

The MSDS for these products have been added to each product datasheet. They should appear on the website within the next few hours (time for the cache to be regenerated).

I hope this information is helpful...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Brain - Cerebellum)
Specification Brain - Cerebellum
Fixative Formaldehyde
Antigen retrieval step Heat mediated
Permeabilization No

Ms. Philippa Hepplewhite

Verified customer

Submitted Jun 23 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - other (Isolated Mouse Liver Mitochondria)
Loading amount 20 µg
Specification Isolated Mouse Liver Mitochondria
Gel Running Conditions Reduced Denaturing (10% Bis Tris)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Dec 05 2011