GRIM19 overexpression 293T lysate (whole cell) (ab94271)

Overview

  • Product nameGRIM19 overexpression 293T lysate (whole cell)
  • Description
    GRIM19 overexpression lysate
  • General notesab94271 is a 293T cell transfected lysate in which Human GRIM19 has been transiently over-expressed using a pCMV-GRIM19 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
  • Tested applicationsSuitable for: WBmore details

Properties

  • BackgroundFunction: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Involved in the interferon/all-trans-retinoic acid (IFN/RA) induced cell death. This apoptotic activity is inhibited by interaction with viral IRF1. Prevents the transactivation of STAT3 target genes. May play a role in CARD15-mediated innate mucosal responses and serve to regulate intestinal epithelial cell responses to microbes. Tissue specificity: Widely expressed, with highest expression in heart, skeletal muscle, liver, kidney and placenta. In intestinal mucosa, down-regulated in areas involved in Crohn disease and ulcerative colitis. Disease: Defects in NDUFA13 may be a cause of susceptibility to Hurthle cell thyroid carcinoma (HCTC) [MIM:607464]. Hurthle cell thyroid carcinoma accounts for approximately 3% of all thyroid cancers. Although they are classified as variants of follicular neoplasms, they are more often multifocal and somewhat more aggressive and are less likely to take up iodine than are other follicular neoplasms. Similarity: Belongs to the complex I NDUFA13 subunit family. Developmental stage: Expressed in numerous fetal tissues.

Associated products

Applications

Our Abpromise guarantee covers the use of ab94271 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

GRIM19 overexpression 293T lysate (whole cell) images

  • ab94271 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-GRIM19 antibody (ab88094) at 1/500 dilution

    Lane 1 : GRIM19 overexpression 293T lysate (whole cell) (ab94271)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References for GRIM19 overexpression 293T lysate (whole cell) (ab94271)

ab94271 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"