Overview

  • Product name
  • Description
    Rabbit polyclonal to GRIP1
  • Specificity
    This antibody is raised against glutamate receptor-interacting protein 1
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat, Elephant
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 725-775 of Human GRIP1 (NP_066973.2).

  • Positive control
    • 293T whole cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH 7 to 8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab168848 was affinity purified using an epitope specific to GRIP1 immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab168848 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 122 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function
    May play a role as a localized scaffold for the assembly of a multiprotein signaling complex and as mediator of the trafficking of its binding partners at specific subcellular location in neurons.
  • Sequence similarities
    Contains 7 PDZ (DHR) domains.
  • Domain
    PDZ 6 mediates interaction with the PDZ recognition motif of EFNB1 and EPHB2 and with the C-terminus of PPFIA1 and PPFIA4. PDZ 4 and PDZ 5 mediate interaction with the C-terminus of GRIA2 and GRIA3. PDZ 4, PDZ 5 and PDZ 6 mediate homomultimers. PDZ 7 mediates interaction with PDZ domain of GRASP1. PDZ 7 domain binds CSPG4. PDZ 6 mediates interaction with the C-terminus of liprins-alpha. PDZ 1, PDZ 2 and PDZ 3 mediate interaction with the PDZ-binding motif of FRAS1 (By similarity). PDZ 4 and PDZ 5 mediate interaction with PRLHR.
  • Cellular localization
    Cytoplasmic vesicle. Endoplasmic reticulum. Cell junction > synapse > postsynaptic cell membrane. Cytoplasmic and membrane-associated with vesicles, peri-Golgi complexes and endoplasmic reticulum. Enriched in post-synaptic plasma membrane and post-synaptic densities.
  • Information by UniProt
  • Database links
  • Alternative names
    • as GRIP antibody
    • Glutamate receptor-interacting protein 1 antibody
    • GRIP-1 antibody
    • Grip1 antibody
    • GRIP1_HUMAN antibody
    see all

Images

  • All lanes : Anti-GRIP1 antibody (ab168848) at 0.1 µg/ml

    Lane 1 : 293T whole cell lysate
    Lane 2 : HeLa whole cell lysate
    Lane 3 : Jurkat whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique

    Predicted band size : 122 kDa


    Exposure time : 3 minutes
  • Detection of GRIP1 in Immunoprecipitates of 293T whole cell lysate (1 mg for IP, 20% of IP loaded) using ab168848 at 6 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.
    Detection: Chemiluminescence with an exposure time of 30 seconds.

References

ab168848 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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