Anti-GRIP1 antibody [GRP01] (ab44970)

Overview

  • Product name
    Anti-GRIP1 antibody [GRP01]
    See all GRIP1 primary antibodies
  • Description
    Mouse monoclonal [GRP01] to GRIP1
  • Specificity
    This antibody is raised against glutamate receptor-interacting protein 1
  • Tested applications
    Suitable for: ICC, IP, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant GRIP1 protein (Human)

Properties

Applications

Our Abpromise guarantee covers the use of ab44970 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
IP 1/400.
WB 1/200. Detects a band of approximately 160 kDa.

Target

  • Function
    May play a role as a localized scaffold for the assembly of a multiprotein signaling complex and as mediator of the trafficking of its binding partners at specific subcellular location in neurons.
  • Sequence similarities
    Contains 7 PDZ (DHR) domains.
  • Domain
    PDZ 6 mediates interaction with the PDZ recognition motif of EFNB1 and EPHB2 and with the C-terminus of PPFIA1 and PPFIA4. PDZ 4 and PDZ 5 mediate interaction with the C-terminus of GRIA2 and GRIA3. PDZ 4, PDZ 5 and PDZ 6 mediate homomultimers. PDZ 7 mediates interaction with PDZ domain of GRASP1. PDZ 7 domain binds CSPG4. PDZ 6 mediates interaction with the C-terminus of liprins-alpha. PDZ 1, PDZ 2 and PDZ 3 mediate interaction with the PDZ-binding motif of FRAS1 (By similarity). PDZ 4 and PDZ 5 mediate interaction with PRLHR.
  • Cellular localization
    Cytoplasmic vesicle. Endoplasmic reticulum. Cell junction > synapse > postsynaptic cell membrane. Cytoplasmic and membrane-associated with vesicles, peri-Golgi complexes and endoplasmic reticulum. Enriched in post-synaptic plasma membrane and post-synaptic densities.
  • Information by UniProt
  • Database links
  • Alternative names
    • as GRIP antibody
    • Glutamate receptor-interacting protein 1 antibody
    • GRIP-1 antibody
    • Grip1 antibody
    • GRIP1_HUMAN antibody
    see all

Anti-GRIP1 antibody [GRP01] images

  • ab44970 staining GRIP1 in CHO Cells stably transfected with human GRIP1 by Immunocytochemistry/ Immunofluorescence. Cells were fixed in paraformaldehyde and permeabilized in 0.5% Triton X-100 prior to blocking in 10% serum for 1 hour at room temperature. The primary antibody was diluted 1/500 in PBS, 0.5% Triton, 10% FCS and incubated with the sample for 2 hours. The secondary antibody was Alexa Fluor® 488-conjugated Goat anti-Mouse polyclonal, diluted 1/400.

    See Abreview

References for Anti-GRIP1 antibody [GRP01] (ab44970)

ab44970 has not yet been referenced specifically in any publications.

Product Wall

Thank you for submitting an Abreview of ab44970. As you may have noticed, your review has now been published on our website.

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Application
Western blot
Sample
Human Cell lysate - whole cell (Stably transfected CHO cells expressing human GRIP)
Loading amount
20 µg
Specification
Stably transfected CHO cells expressing human GRIP
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Verified customer

Submitted Nov 28 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Human Cultured Cells (CHO Cells stably transfected with human Grip1)
Specification
CHO Cells stably transfected with human Grip1
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
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Verified customer

Submitted Apr 30 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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