Anti-GRIP1 antibody [GRP01] (ab44970)

Overview

  • Product nameAnti-GRIP1 antibody [GRP01]
    See all GRIP1 primary antibodies
  • Description
    Mouse monoclonal [GRP01] to GRIP1
  • SpecificityThis antibody is raised against glutamate receptor-interacting protein 1
  • Tested applicationsSuitable for: ICC, IP, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant GRIP1 protein (Human)

Properties

Applications

Our Abpromise guarantee covers the use of ab44970 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
IP 1/400.
WB 1/200. Detects a band of approximately 160 kDa.

Target

  • FunctionMay play a role as a localized scaffold for the assembly of a multiprotein signaling complex and as mediator of the trafficking of its binding partners at specific subcellular location in neurons.
  • Sequence similaritiesContains 7 PDZ (DHR) domains.
  • DomainPDZ 6 mediates interaction with the PDZ recognition motif of EFNB1 and EPHB2 and with the C-terminus of PPFIA1 and PPFIA4. PDZ 4 and PDZ 5 mediate interaction with the C-terminus of GRIA2 and GRIA3. PDZ 4, PDZ 5 and PDZ 6 mediate homomultimers. PDZ 7 mediates interaction with PDZ domain of GRASP1. PDZ 7 domain binds CSPG4. PDZ 6 mediates interaction with the C-terminus of liprins-alpha. PDZ 1, PDZ 2 and PDZ 3 mediate interaction with the PDZ-binding motif of FRAS1 (By similarity). PDZ 4 and PDZ 5 mediate interaction with PRLHR.
  • Cellular localizationCytoplasmic vesicle. Endoplasmic reticulum. Cell junction > synapse > postsynaptic cell membrane. Cytoplasmic and membrane-associated with vesicles, peri-Golgi complexes and endoplasmic reticulum. Enriched in post-synaptic plasma membrane and post-synaptic densities.
  • Information by UniProt
  • Database links
  • Alternative names
    • as GRIP antibody
    • Glutamate receptor-interacting protein 1 antibody
    • GRIP-1 antibody
    • Grip1 antibody
    • GRIP1_HUMAN antibody
    see all

Anti-GRIP1 antibody [GRP01] images

  • ab44970 staining GRIP1 in CHO Cells stably transfected with human GRIP1 by Immunocytochemistry/ Immunofluorescence. Cells were fixed in paraformaldehyde and permeabilized in 0.5% Triton X-100 prior to blocking in 10% serum for 1 hour at room temperature. The primary antibody was diluted 1/500 in PBS, 0.5% Triton, 10% FCS and incubated with the sample for 2 hours. The secondary antibody was Alexa Fluor® 488-conjugated Goat anti-Mouse polyclonal, diluted 1/400.

    See Abreview

References for Anti-GRIP1 antibody [GRP01] (ab44970)

ab44970 has not yet been referenced specifically in any publications.

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Application Western blot
Sample Human Cell lysate - whole cell (Stably transfected CHO cells expressing human GRIP)
Loading amount 20 µg
Specification Stably transfected CHO cells expressing human GRIP
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Verified customer

Submitted Nov 28 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Human Cultured Cells (CHO Cells stably transfected with human Grip1)
Specification CHO Cells stably transfected with human Grip1
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
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Verified customer

Submitted Apr 30 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"