Recombinant Anti-GRK1 antibody [EPR2039(2)] (ab108502)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2039(2)] to GRK1
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-GRK1 antibody [EPR2039(2)]
See all GRK1 primary antibodies -
Description
Rabbit monoclonal [EPR2039(2)] to GRK1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Y79 and JAR cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2039(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab108502 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa). |
Target
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Function
Retina-specific kinase involved in the signal turnoff via phosphorylation of rhodopsin (RHO), the G protein- coupled receptor that initiates the phototransduction cascade. This rapid desensitization is essential for scotopic vision and permits rapid adaptation to changes in illumination. -
Tissue specificity
Retinal-specific. Expressed in rods and cones cells. -
Involvement in disease
Night blindness, congenital stationary, Oguchi type 2 -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain.
Contains 1 RGS domain. -
Post-translational
modificationsAutophosphorylated, Ser-21 is a minor site of autophosphorylation compared to Ser-491 and Thr-492 (By similarity). Phosphorylation at Ser-21 is regulated by light and activated by cAMP.
Farnesylation is required for full activity. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 131890 Human
- Entrez Gene: 6011 Human
- Entrez Gene: 24013 Mouse
- Entrez Gene: 81760 Rat
- Omim: 180381 Human
- SwissProt: Q15835 Human
- SwissProt: Q8WTQ7 Human
- SwissProt: Q9WVL4 Mouse
see all -
Alternative names
- EC=2.7.11.14 antibody
- G protein-coupled receptor kinase 1 antibody
- GPRK1 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab108502 has been referenced in 2 publications.
- Pu Y et al. GOLM1 restricts colitis and colon tumorigenesis by ensuring Notch signaling equilibrium in intestinal homeostasis. Signal Transduct Target Ther 6:148 (2021). PubMed: 33850109
- Long D et al. VEGF/VEGFR2 blockade does not cause retinal atrophy in AMD-relevant models. JCI Insight 3:N/A (2018). PubMed: 29769445