Overview

  • Product nameAnti-GRK1 (phospho S21) antibody
    See all GRK1 primary antibodies
  • Description
    Rabbit polyclonal to GRK1 (phospho S21)
  • Specificityab74033 detects endogenous levels of GRK1 only when phosphorylated at serine 21.
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phosphopeptide derived from human GRK1 around the phosphorylation site of serine 21 (R-G-SP-F-D).

  • Positive control
    • COS7 cell extracts treated with TNF (20ng/ml, 5mins) and human brain tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab74033 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
ELISA 1/10000.
IHC-P 1/50 - 1/100.

Target

  • FunctionRetina-specific kinase involved in the signal turnoff via phosphorylation of rhodopsin (RHO), the G protein- coupled receptor that initiates the phototransduction cascade. This rapid desensitization is essential for scotopic vision and permits rapid adaptation to changes in illumination.
  • Tissue specificityRetinal-specific. Expressed in rods and cones cells.
  • Involvement in diseaseNight blindness, congenital stationary, Oguchi type 2
  • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 protein kinase domain.
    Contains 1 RGS domain.
  • Post-translational
    modifications
    Autophosphorylated, Ser-21 is a minor site of autophosphorylation compared to Ser-491 and Thr-492 (By similarity). Phosphorylation at Ser-21 is regulated by light and activated by cAMP.
    Farnesylation is required for full activity.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • EC=2.7.11.14 antibody
    • G protein-coupled receptor kinase 1 antibody
    • GPRK1 antibody
    • Grk1 antibody
    • Rhodopsin kinase antibody
    • RHOK antibody
    • RK antibody
    • RK_HUMAN antibody
    see all

Anti-GRK1 (phospho S21) antibody images

  • ab74033 at 1/50 dilution staining GRK1 in human brain by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.
  • All lanes : Anti-GRK1 (phospho S21) antibody (ab74033) at 1/500 dilution

    Lane 1 : COS7 cell extracts, treated with TNF (20ng/ml, 5mins)
    Lane 2 : COS7 cell extracts, treated with TNF (20ng/ml, 5mins) with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 63 kDa
    Observed band size : 63 kDa

References for Anti-GRK1 (phospho S21) antibody (ab74033)

ab74033 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab74033.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"