Overview

  • Product name
  • Description
    Rabbit polyclonal to GRK2
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 1-358 of Human GRK2 (UniProt ID: P25098).

  • Positive control
    • Raji whole cell lysate; HeLa cells; Human Cal27 xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab153712 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 80 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them. Key regulator of LPAR1 signaling. Competes with RALA for binding to LPAR1 thus affecting the signaling properties of the receptor. Desensitizes LPAR1 and LPAR2 in a phosphorylation-independent manner.
  • Tissue specificity
    Expressed in peripheral blood leukocytes.
  • Sequence similarities
    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.
    Contains 1 RGS domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADRBK1 antibody
    • Adrenergic beta receptor kinase 1 antibody
    • ARBK1_HUMAN antibody
    • BARK antibody
    • BARK1 antibody
    • Beta adrenergic receptor kinase 1 antibody
    • Beta ARK 1 antibody
    • Beta ARK1 antibody
    • Beta-adrenergic receptor kinase 1 antibody
    • Beta-ARK-1 antibody
    • FLJ16718 antibody
    • G protein coupled receptor kinase 2 antibody
    • G-protein coupled receptor kinase 2 antibody
    • GRK2 antibody
    see all

Images

  • Anti-GRK2 antibody (ab153712) at 1/1000 dilution + Raji whole cell lysate at 30 µg

    Predicted band size : 80 kDa
  • Immunohistochemical analysis of paraffin embedded Human Cal27 xenograft tissue, labeling GRK2 using ab153712 at a 1/500 dilution.
  • Immunofluorescence analysis of methanol fixed HeLa cells, labeling GRK2 using ab153712 at a 1/500 dilution. Lower image costained with Hoerchst 33342.

References

ab153712 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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