Overview

  • Product nameAnti-GRK2 antibody
    See all GRK2 primary antibodies
  • Description
    Rabbit polyclonal to GRK2
  • SpecificityThis antibody detects endogenous levels of total GRK2 protein.
  • Tested applicationsSuitable for: ICC/IF, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide (Human) from an internal region of GRK2.

  • Positive control
    • Extracts from HuvEc cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab64858 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 80 kDa).
ELISA 1/20000.

Target

  • FunctionSpecifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them. Key regulator of LPAR1 signaling. Competes with RALA for binding to LPAR1 thus affecting the signaling properties of the receptor. Desensitizes LPAR1 and LPAR2 in a phosphorylation-independent manner.
  • Tissue specificityExpressed in peripheral blood leukocytes.
  • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.
    Contains 1 RGS domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADRBK1 antibody
    • Adrenergic beta receptor kinase 1 antibody
    • ARBK1_HUMAN antibody
    • BARK antibody
    • BARK1 antibody
    • Beta adrenergic receptor kinase 1 antibody
    • Beta ARK 1 antibody
    • Beta ARK1 antibody
    • Beta-adrenergic receptor kinase 1 antibody
    • Beta-ARK-1 antibody
    • FLJ16718 antibody
    • G protein coupled receptor kinase 2 antibody
    • G-protein coupled receptor kinase 2 antibody
    • GRK2 antibody
    see all

Anti-GRK2 antibody images

  • All lanes : Anti-GRK2 antibody (ab64858) at 1/500 dilution

    Lane 1 : Extracts from HuvEc cells
    Lane 2 : Extracts from HuvEc cells with immunising peptide


    Predicted band size : 80 kDa
    Observed band size : 80 kDa
    Additional bands at : ~35 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab64858 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64858, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-GRK2 antibody (ab64858)

ab64858 has not yet been referenced specifically in any publications.

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