Anti-GRK2 (phospho S29) antibody (ab58520)

Overview

  • Product nameAnti-GRK2 (phospho S29) antibody
    See all GRK2 primary antibodies
  • Description
    Rabbit polyclonal to GRK2 (phospho S29)
  • SpecificityThis antibody detects endogenous levels of GRK2 only when phosphorylated at serine 29.
  • Tested applicationsSuitable for: IHC-FoFr, IHC-P, ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthesized phosphopeptide derived from human GRK2 around the phosphorylation site of serine 29 (R-A-SP-K-K).

  • Positive control
    • IHC-P: human breast carcinoma tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThe antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab58520 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr 1/1000.
IHC-P Use at an assay dependent concentration.
ELISA 1/10000.
WB 1/500 - 1/1000. Predicted molecular weight: 80 kDa.

Target

  • FunctionSpecifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them. Key regulator of LPAR1 signaling. Competes with RALA for binding to LPAR1 thus affecting the signaling properties of the receptor. Desensitizes LPAR1 and LPAR2 in a phosphorylation-independent manner.
  • Tissue specificityExpressed in peripheral blood leukocytes.
  • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.
    Contains 1 RGS domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADRBK1 antibody
    • Adrenergic beta receptor kinase 1 antibody
    • ARBK1_HUMAN antibody
    • BARK antibody
    • BARK1 antibody
    • Beta adrenergic receptor kinase 1 antibody
    • Beta ARK 1 antibody
    • Beta ARK1 antibody
    • Beta-adrenergic receptor kinase 1 antibody
    • Beta-ARK-1 antibody
    • FLJ16718 antibody
    • G protein coupled receptor kinase 2 antibody
    • G-protein coupled receptor kinase 2 antibody
    • GRK2 antibody
    see all

Anti-GRK2 (phospho S29) antibody images

  • This image shows human breast carcinoma tissue stained with ab58520 at a 1/50 dilution. Right hand image: tissue treated with the immunising phosphopeptide. Left hand image; without the immunising phosphopeptide.
  • Immunohistochemical analysis of adult mouse brain tissue, staining GRK2 (phospho S29) with ab58520.

    Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100and blocked with 10% horse serum for 1 hour at 20°C;. Samples were incubated with primary antibody (1/1000 in 10% horse serum in PBS + 0.1% Triton X-100) for 16 hours at 4°C. An AlexaFluor®488-conjugated donkey anti-rabbit polyclonal IgG (1/1000) was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-GRK2 (phospho S29) antibody (ab58520)

    Lane 1 : Jurkat cells treated with EGF
    Lane 2 : Jurkat cells treated with EGF with phospho peptide


    Predicted band size : 80 kDa

References for Anti-GRK2 (phospho S29) antibody (ab58520)

ab58520 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (adult mouse brain)
Specification adult mouse brain
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization Yes - 0.1% Triton X-100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Username

Dr. Hai-Ying Mary Cheng

Verified customer

Submitted Dec 17 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"