Anti-GRK2 (phospho S29) antibody (ab58520)
Key features and details
- Rabbit polyclonal to GRK2 (phospho S29)
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GRK2 (phospho S29) antibody
See all GRK2 primary antibodies -
Description
Rabbit polyclonal to GRK2 (phospho S29) -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide corresponding to Human GRK2 aa 1-100 (phospho S29).
Database link: P25098 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab58520 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use at an assay dependent concentration.
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WB | (1) |
1/500 - 1/1000. Predicted molecular weight: 80 kDa.
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Notes |
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IHC-P
Use at an assay dependent concentration. |
WB
1/500 - 1/1000. Predicted molecular weight: 80 kDa. |
Target
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Function
Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them. Key regulator of LPAR1 signaling. Competes with RALA for binding to LPAR1 thus affecting the signaling properties of the receptor. Desensitizes LPAR1 and LPAR2 in a phosphorylation-independent manner. -
Tissue specificity
Expressed in peripheral blood leukocytes. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 protein kinase domain.
Contains 1 RGS domain. - Information by UniProt
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Database links
- Entrez Gene: 156 Human
- Entrez Gene: 25238 Rat
- Omim: 109635 Human
- SwissProt: P25098 Human
- SwissProt: P26817 Rat
- Unigene: 83636 Human
- Unigene: 13010 Rat
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Alternative names
- ADRBK1 antibody
- Adrenergic beta receptor kinase 1 antibody
- ARBK1_HUMAN antibody
see all
Images
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All lanes : Anti-GRK2 (phospho S29) antibody (ab58520)
Lane 1 : Jurkat cells treated with EGF
Lane 2 : Jurkat cells treated with EGF with phospho peptide
Predicted band size: 80 kDa -
This image shows human breast carcinoma tissue stained with ab58520 at a 1/50 dilution. Right hand image: tissue treated with the immunising phosphopeptide. Left hand image; without the immunising phosphopeptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab58520 has been referenced in 1 publication.
- Schreckenberg R et al. Adverse Effects on ß-Adrenergic Receptor Coupling: Ischemic Postconditioning Failed to Preserve Long-Term Cardiac Function. J Am Heart Assoc 6:N/A (2017). PubMed: 29273639