Purification notesThe antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
FunctionSpecifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them. Key regulator of LPAR1 signaling. Competes with RALA for binding to LPAR1 thus affecting the signaling properties of the receptor. Desensitizes LPAR1 and LPAR2 in a phosphorylation-independent manner.
Tissue specificityExpressed in peripheral blood leukocytes.
Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 PH domain. Contains 1 protein kinase domain. Contains 1 RGS domain.
This image shows human breast carcinoma tissue stained with ab58520 at a 1/50 dilution. Right hand image: tissue treated with the immunising phosphopeptide. Left hand image; without the immunising phosphopeptide.
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-GRK2 (phospho S29) antibody (ab58520)This image is courtesy of an Abreview submitted by Dr Hai-Ying Mary Cheng
Immunohistochemical analysis of adult mouse brain tissue, staining GRK2 (phospho S29) with ab58520.
Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100and blocked with 10% horse serum for 1 hour at 20°C;. Samples were incubated with primary antibody (1/1000 in 10% horse serum in PBS + 0.1% Triton X-100) for 16 hours at 4°C. An AlexaFluor®488-conjugated donkey anti-rabbit polyclonal IgG (1/1000) was used as the secondary antibody.