Overview

  • Product nameAnti-GRK4 antibodySee all GRK4 primary antibodies ...
  • Description
    Mouse monoclonal to GRK4
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Recombinant Fragment
    Predicted to work with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 36-145 of Human GRK4

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • Clonality Monoclonal
  • IsotypeIgG2b
  • Light chain typekappa
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab55139 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
  • Application notesWB: Use at a concentration of 1-5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionSpecifically phosphorylates the activated forms of G protein-coupled receptors. GRK4-alpha can phosphorylate rhodopsin and its activity is inhibited by calmodulin; the other three isoforms do not phosphorylate rhodopsin and do not interact with calmodulin. GRK4-alpha and GRK4-gamma phosphorylate DRD3. Phosphorylates ADRB2.
    • Tissue specificityIsoform 1, isoform 2, isoform 3, and isoform 4 are expressed in testis. Isoform 4 is expressed in myometrium.
    • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 1 protein kinase domain.
      Contains 1 RGS domain.
    • Post-translational
      modifications
      Palmitoylated.
    • Cellular localizationCytoplasm. Cytoplasm > cell cortex. Both at the cell surface and dispersed in the cytoplasm under basal conditions. Receptor stimulation results in the internalization of GRK4 to the perinuclear area, where co-localization with DRD3 is observed strongly at 5 and 15 minutes. DRD3 and GRK4 co-localize in lipid rafts of renal proximal tubule cells.
    • Target information above from: UniProt accession P32298 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • G protein coupled receptor kinase 4 antibody
      • G protein-coupled receptor kinase 2 (Drosophila)-like antibody
      • G protein-coupled receptor kinase 4 antibody
      • G protein-coupled receptor kinase GRK4 antibody
      • G proteinm coupled receptor kinase GRK4 antibody
      • GPRK2L antibody
      • GPRK4 antibody
      • GRK4 antibody
      • GRK4_HUMAN antibody
      • GRK4a antibody
      • ITI1 antibody
      see all

    Anti-GRK4 antibody images

    • Western blot against tagged recombinant protein immunogen using ab55139 GRK4 antibody at 1ug/ml. Predicted band size of immunogen is 38 kDa.

      This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    References for Anti-GRK4 antibody (ab55139)

    ab55139 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"