Overview

  • Product nameAnti-GRK6 antibody
    See all GRK6 primary antibodies
  • Description
    Rabbit polyclonal to GRK6
  • Specificityab64915 detects endogenous levels of total GRK6 protein.
  • Tested applicationsSuitable for: IHC-P, WB, ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from the N terminal of Human GRK6

  • Positive control
    • HeLa cells, and extracts from Jurkat, COLO205 and HUVEC cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab64915 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa).
ICC/IF 1/500 - 1/1000.
ELISA 1/10000.

Target

  • FunctionSpecifically phosphorylates the activated forms of G protein-coupled receptors.
  • Tissue specificityWidely expressed.
  • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 protein kinase domain.
    Contains 1 RGS domain.
  • Post-translational
    modifications
    It is uncertain whether palmitoylation is on Cys-561 and/or Cys-562 and/or Cys-565.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • FormThere are 2 isoforms produced by alternative splicing.
  • Alternative names
    • FLJ32135 antibody
    • G protein coupled receptor kinase 6 antibody
    • G protein coupled receptor kinase GRK6 antibody
    • G protein-coupled receptor kinase 6 antibody
    • G protein-coupled receptor kinase GRK6 antibody
    • Gprk6 antibody
    • Grk6 antibody
    • GRK6_HUMAN antibody
    see all

Anti-GRK6 antibody images

  • All lanes : Anti-GRK6 antibody (ab64915) at 1/500 dilution

    Lane 1 : Extracts from Jurkat cells
    Lane 2 : Extracts from COLO205 cells
    Lane 3 : Extracts from HUVEC cells
    Lane 4 : Extracts from HUVEC cells with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 66 kDa
    Observed band size : 66 kDa
    Additional bands at : 20 kDa,26 kDa. We are unsure as to the identity of these extra bands.
  • ab64915, at a 1/500 dilution, staining Human GRK6 in HeLa cells, by Immunofluorescence, with (+) or without (-) immunising peptide.
  • IHC image of ab64915 staining in human normal heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab64915, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-GRK6 antibody (ab64915)

ab64915 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (brain)
Loading amount 20 µg
Specification brain
Gel Running Conditions Reduced Denaturing (4-12% gradient)
Blocking step LiCor buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
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Submitted Mar 31 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"