For the best experience on the Abcam website please upgrade to a modern browser such as
Rabbit polyclonal to GRK6
ab64915 detects endogenous levels of total GRK6 protein.
Predicted to work with:
Synthetic peptide derived from the N terminal of Human GRK6
HeLa cells, and extracts from Jurkat, COLO205 and HUVEC cells.
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg 2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
Immunogen affinity purified
ab64915 was affinity-purified from rabbit antiserum by affinity-chromatography using an epitope-specific immunogen.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
1/500 - 1/1000. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa).
1/500 - 1/1000.
Specifically phosphorylates the activated forms of G protein-coupled receptors.
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.
Contains 1 AGC-kinase C-terminal domain. Contains 1 protein kinase domain. Contains 1 RGS domain.
It is uncertain whether palmitoylation is on Cys-561 and/or Cys-562 and/or Cys-565.
Information by UniProt
There are 2 isoforms produced by alternative splicing.
G protein coupled receptor kinase 6 antibody
G protein coupled receptor kinase GRK6 antibody
Western blot - GRK6 antibody (ab64915)
All lanes : Anti-GRK6 antibody (ab64915) at 1/500 dilution Lane 1 : Extracts from Jurkat cells Lane 2 : Extracts from COLO205 cells Lane 3 : Extracts from HUVEC cells Lane 4 : Extracts from HUVEC cells with immunising peptide at 10 µg Lysates/proteins at 30 µg per lane. Predicted band size : 66 kDa Observed band size : 66 kDa Additional bands at : 20 kDa,26 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence - GRK6 antibody (ab64915)
ab64915, at a 1/500 dilution, staining Human GRK6 in HeLa cells, by Immunofluorescence, with (+) or without (-) immunising peptide.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-GRK6 antibody(ab64915)
IHC image of ab64915 staining in human normal heart formalin fixed paraffin embedded tissue section, performed on a Leica Bond
TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab64915, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"