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Rabbit polyclonal to GRK6 - Carboxyterminal end
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected within aa 500~600 of human GRK6.
HeLa cell lysate, Mouse heart tissue lysate.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.09% Sodium Azide
Concentration information loading...
Protein G purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa).
1/50 - 1/100.
Specifically phosphorylates the activated forms of G protein-coupled receptors.
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.
Contains 1 AGC-kinase C-terminal domain. Contains 1 protein kinase domain. Contains 1 RGS domain.
It is uncertain whether palmitoylation is on Cys-561 and/or Cys-562 and/or Cys-565.
Information by UniProt
There are 2 isoforms produced by alternative splicing.
G protein coupled receptor kinase 6 antibody
G protein coupled receptor kinase GRK6 antibody
Western blot - GRK6 antibody (ab38291)
All lanes : Anti-GRK6 antibody - Carboxyterminal end (ab38291) at 1/100 dilution Lane 1 : HeLa cell lysate. Lane 2 : Mouse heart tissue lysate. Secondary All lanes : HRP-anti-rabbit. Developed using the ECL technique. Predicted band size: 66 kDa Observed band size: 66 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GRK6 antibody (ab38291)
ab38291 (1/50) staining human cancer tissue by immunohistochemistry, formalin-fixed, paraffin-embedded tissue. The breast carcinoma tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
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